Jeon Sohee, Baek Jiwon, Lee Won Ki
Department of Ophthalmology, Seoul St. Mary's Hospital, The Catholic University of Korea College of Medicine, Seoul, South Korea.
Invest Ophthalmol Vis Sci. 2017 Jan 1;58(1):651-659. doi: 10.1167/iovs.16-20409.
We evaluate the expression of Gli1 in human epiretinal membranes (ERM) and correlate this with clinical data.
We prospectively recruited patients with ERM. A total of 33 human ERM specimens were immunolabeled with anti-Gli1 antibody and the number of total cells/hyperfield (HF), Gli1(+) cells/HF, and the percentage of Gli1(+) cells/total cells were calculated. We evaluated the interrelationship of cellular properties and clinical findings, such as presence of diabetic retinopathy (DR), retinal breaks, intraocular inflammation, central foveal thickness, maximal retinal thickness, retinal contraction, lamellar holes, pseudoholes, the attenuation or absence of an inner segment/outer segment (IS/OS) junction/external limiting membrane (ELM), cystic changes, and paravascular inner retinal defects.
Among 33 specimens, 25 specimens (75.8%) showed nuclear Gli1 expression. The mean Gli1(+) cells/total cells was 54.0 ± 36.7% (range, 0%-92.8%). There was significantly higher expression of Gli1(+) cells in ERM specimens from patients with DR (P = 0.014), and lower expression from patients with retinal breaks (P = 0.022). Epiretinal membrane specimens from patients with alteration of IS/OS junction/ELM or cystic changes on OCT showed higher percentage of Gli1(+) cells/total cells.
Gli1 expression was detected in most ERM specimens. Patients who had DR or OCT findings indicating chronic retinal insults showed higher Gli1 expression. Gli1 may have a role in the pathogenesis of ERM after chronic retinal insults.
我们评估Gli1在人视网膜前膜（ERM）中的表达，并将其与临床数据相关联。
我们前瞻性招募了ERM患者。总共33个人类ERM标本用抗Gli1抗体进行免疫标记，并计算每个高倍视野（HF）中的总细胞数、Gli1(+)细胞数以及Gli1(+)细胞占总细胞的百分比。我们评估了细胞特性与临床发现之间的相互关系，如糖尿病视网膜病变（DR）的存在、视网膜裂孔、眼内炎症、中心凹厚度、最大视网膜厚度、视网膜收缩、板层孔、假性孔、内节/外节（IS/OS）交界处/外界膜（ELM）的减弱或缺失、囊性改变以及视网膜血管旁内层缺损。
在33个标本中，25个标本（75.8%）显示出核Gli1表达。Gli1(+)细胞占总细胞的平均百分比为54.0 ± 36.7%（范围为0%-92.8%）。DR患者的ERM标本中Gli1(+)细胞表达显著更高（P = 0.014），而视网膜裂孔患者的表达较低（P = 0.022）。光学相干断层扫描（OCT）显示IS/OS交界处/ELM改变或囊性改变的患者的视网膜前膜标本中，Gli1(+)细胞占总细胞的百分比更高。
在大多数ERM标本中检测到Gli1表达。患有DR或OCT结果表明存在慢性视网膜损伤的患者显示出更高的Gli1表达。Gli1可能在慢性视网膜损伤后的ERM发病机制中起作用。
