Tien Luu Viet, Yamamoto Manabu, Tagami Mizuki, Honda Shigeru
Department of Ophthalmology and Visual Sciences, Graduate School of Medicine, Osaka Metropolitan University, 1-4-3 Asahimachi, Abeno-ku, Osaka, 545-8585, Japan.
Sci Rep. 2025 Jul 2;15(1):22767. doi: 10.1038/s41598-025-05199-3.
Myofibroblasts were recently identified as the most critical cells responsible for epiretinal membrane contraction, however the myofibroblast number in different types of membranes may differ substantially. In this retrospective study, we compare myofibroblast distribution and density in idiopathic epiretinal membranes (iERMs) and epiretinal membranes (ERMs) in proliferative diabetic retinopathy (PDR). Samples (n = 42) from iERM and from thin ERM, ERM, and epiretinal fibrovascular membrane (FVM) in PDR (thin ERM/PDR, ERM/PDR, and FVM/PDR, respectively) were collected via a standard 27-gauge vitrectomy. They were analyzed using flat-mount immunohistochemical staining with α-smooth muscle actin. Cell densities (in cells/mm) for iERM, thin ERM/PDR, ERM/PDR, and FVM/PDR were 339.00 ± 256.76, 12.39 ± 7.46, 114.22 ± 40.51, and 844.15 ± 323.21, respectively, and significantly different (P < 0.001). Myofibroblast densities (in cells/mm) in these groups were 39.48 ± 46.51, 0.65 ± 0.95, 9.22 ± 12.08, and 303.43 ± 57.21, respectively, and significantly different (P < 0.001). Myofibroblast density in iERM was higher than that of thin ERM/PDR (P < 0.001), higher but not significantly different from ERM/PDR (P = 0.077), and lower than that of FVM/PDR (P < 0.001). The myofibroblasts/total cell ratios in these groups were 12.66% ± 12.38%, 5.89% ± 9.78%, 9.55% ± 10.86%, and 40.35% ± 14.37%, respectively, and significantly different (P < 0.001). Myofibroblast density was strongly correlated with cell density and the myofibroblast/total cell ratio (P < 0.001).
肌成纤维细胞最近被确定为视网膜前膜收缩的最关键细胞，然而不同类型膜中的肌成纤维细胞数量可能有很大差异。在这项回顾性研究中，我们比较了特发性视网膜前膜（iERM）以及增殖性糖尿病视网膜病变（PDR）中的视网膜前膜（ERM）和视网膜前纤维血管膜（FVM）中肌成纤维细胞的分布和密度。通过标准的27G玻璃体切除术收集来自iERM以及PDR中的薄ERM、ERM和视网膜前纤维血管膜（分别为薄ERM/PDR、ERM/PDR和FVM/PDR）的样本（n = 42）。使用α-平滑肌肌动蛋白进行平铺免疫组织化学染色对其进行分析。iERM、薄ERM/PDR、ERM/PDR和FVM/PDR的细胞密度（细胞数/mm）分别为339.00±256.76、12.39±7.46、114.22±40.51和844.15±323.21，且差异有统计学意义（P < 0.001）。这些组中的肌成纤维细胞密度（细胞数/mm）分别为39.48±46.51、0.65±0.95、9.22±12.08和303.43±57.21，且差异有统计学意义（P < 0.001）。iERM中的肌成纤维细胞密度高于薄ERM/PDR（P < 0.001），高于ERM/PDR但差异无统计学意义（P = 0.077），低于FVM/PDR（P < 0.001）。这些组中的肌成纤维细胞/总细胞比率分别为12.66%±12.38%、5.89%±9.78%、9.55%±10.86%和40.35%±14.37%，且差异有统计学意义（P < 0.001）。肌成纤维细胞密度与细胞密度以及肌成纤维细胞/总细胞比率密切相关（P < 0.001）。
