Buskirk Allen R, Green Rachel
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, MD, USA.
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, MD, USA
Philos Trans R Soc Lond B Biol Sci. 2017 Mar 19;372(1716). doi: 10.1098/rstb.2016.0183.
Ribosomes translate genetic information into polypeptides in several basic steps: initiation, elongation, termination and recycling. When ribosomes are arrested during elongation or termination, the cell's capacity for protein synthesis is reduced. There are numerous quality control systems in place to distinguish between paused ribosomes that need some extra input to proceed and terminally stalled ribosomes that need to be rescued. Here, we discuss similarities and differences in the systems for resolution of pauses and rescue of arrested ribosomes in bacteria and eukaryotes, and how ribosome profiling has transformed our ability to decipher these molecular events.This article is part of the themed issue 'Perspectives on the ribosome'.
起始、延伸、终止和循环利用。当核糖体在延伸或终止过程中停滞时，细胞的蛋白质合成能力就会降低。目前有许多质量控制系统来区分需要一些额外输入才能继续的暂停核糖体和需要被拯救的最终停滞核糖体。在这里，我们讨论了细菌和真核生物中解决暂停和拯救停滞核糖体的系统的异同，以及核糖体分析如何改变了我们解读这些分子事件的能力。本文是主题为“核糖体的视角”特刊的一部分。
