九里香对高胆固醇血症大鼠的降脂特性

Lipid-lowering property of Clausena anisum-olens in hypercholesterolemic rats.

作者信息

Bautista Anbel M, Tan Mario A, Apostol Jovencio M

机构信息

a The Graduate School, University of Santo Tomas , Manila , Philippines.

b Research Center for the Natural and Applied Sciences , University of Santo Tomas , Manila , Philippines.

出版信息

Pharm Biol. 2017 Dec;55(1):833-836. doi: 10.1080/13880209.2016.1260596.

DOI:10.1080/13880209.2016.1260596

PMID:28140741

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6130512/

Abstract

CONTEXT

Clausena anisum-olens (Blanco) Merr. (Rutaceae) is a medicinal shrub which has been reported to have various pharmacological uses. No study regarding the effects of C. anisum-olens on cholesterol-lowering has been reported.

OBJECTIVE

The effects of the ethanol extract of C. anisum-olens leaves on the cholesterol level of hypercholesterolemic rats were evaluated.

MATERIALS AND METHODS

Acute oral toxicity of the extract (175, 550 and 2000 mg/kg) was determined using female Sprague-Dawley rats, as described in OECD 425 Main test guidelines. The lipid-lowering assay utilized 30 male Sprague-Dawley rats divided into five groups (A-E). Triton X-100 was administered to induce hypercholesterolemia. After hypercholesterolemia induction, oral treatment of Atorvastatin and crude ethanol extract was given daily to the treatment groups for 14 days. The total cholesterol, triglycerides, HDL and LDL were determined before induction, after induction, after first week of treatment and after second week of treatment.

RESULTS

Acute oral toxicity showed the crude extract is nontoxic up to 2000 mg/kg. The lipid-lowering assay indicated reduction of serum cholesterol (87.21 ± 5.10 mg/dL), triglycerides (58.09 ± 4.10 mg/dL) and LDL (27.82 ± 4.11 mg/dL) for 200 mg/kw extract. Reduction in serum cholesterol (74.72 ± 3.64 mg/dL), triglycerides (52.79 ± 2.98 mg/dL) and LDL (12.06 ± 5.51 mg/dL) were observed for 400 mg/kg group. The result is comparable to Atorvastatin, which showed serum cholesterol (80.90 ± 9.72 mg/dL), triglycerides (55.94 ± 7.19 mg/dL) and LDL (22.09 ± 7.60 mg/dL) reduction.

DISCUSSION AND CONCLUSION

The crude extract of C. anisum-olens proved to be useful in lowering of cholesterol.

摘要

背景

臭节草（Clausena anisum-olens (Blanco) Merr.，芸香科）是一种药用灌木，据报道具有多种药理用途。尚未有关于臭节草降胆固醇作用的研究报道。

目的

评估臭节草叶乙醇提取物对高胆固醇血症大鼠胆固醇水平的影响。

材料与方法

按照经合组织425号主要试验指南所述，使用雌性斯普拉格-道利大鼠测定提取物（175、550和2000mg/kg）的急性经口毒性。降脂试验使用30只雄性斯普拉格-道利大鼠，分为五组（A - E）。给予 Triton X - 100诱导高胆固醇血症。诱导高胆固醇血症后，治疗组每天口服阿托伐他汀和粗乙醇提取物，持续14天。在诱导前、诱导后、治疗第一周后和治疗第二周后测定总胆固醇、甘油三酯、高密度脂蛋白和低密度脂蛋白。

结果

急性经口毒性表明，粗提取物在高达2000mg/kg时无毒。降脂试验表明，200mg/kg提取物组血清胆固醇（87.21±5.10mg/dL）、甘油三酯（58.09±4.10mg/dL）和低密度脂蛋白（27.82±4.11mg/dL）降低。400mg/kg组血清胆固醇（74.72±3.64mg/dL）、甘油三酯（52.79±2.98mg/dL）和低密度脂蛋白（12.06±5.51mg/dL）降低。结果与阿托伐他汀相当，阿托伐他汀组血清胆固醇（80.90±9.72mg/dL）、甘油三酯（55.94±7.19mg/dL）和低密度脂蛋白（22.09±7.60mg/dL）降低。