Moradi Azam, Ebrahimipour Gholam Hosein, Mohr Kathrin I, Kämpfer Peter, Glaeser Stefanie P, Hennessen Fabienne, Gemperlein Katja, Awal Ram Prasad, Wolf Corinna, Müller Rolf, Wink Joachim
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
Department of Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2017 Feb;67(2):472-478. doi: 10.1099/ijsem.0.001655. Epub 2017 Feb 24.
A novel myxobacterium, strain MSr11462T, was isolated in 2015 from a soil sample collected form Kish Island beach, Persian Gulf, Iran. It displayed general myxobacterial features like Gram-negative staining, rod-shaped vegetative cells, gliding on solid surfaces, microbial lytic activity, fruiting-body-like aggregates and myxospore-like structures. The strain was mesophilic, aerobic and showed a chemoheterotrophic mode of nutrition. It was resistant to many antibiotics like gentamycin, polymyxin, fusidic acid and trimethoprim, and the key fatty acids of whole-cell hydrolysates were iso-C15 : 0, C16 : 0, iso-C17 : 0, C18 : 1, iso-C17 : 1 2-OH, C18 : 1 2-OH, iso-C15 : 0 OAG (O-alkylglycerol) and C16 : 1 OAG. The 16S rRNA gene sequence showed highest similarity (98.6 %) to Racemicystis crocea strain MSr9521T (GenBank accession no. KT591707). The phylogenetic analysis based on 16S rRNA gene sequences and matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) spectroscopy data supports a novel species of the family Polyangiaceae and the genus Racemicystis. DNA-DNA hybridization showed only about 50 % similarity between the novel strain and the phylogenetically closest species, Racemicystis. crocea MSr9521T. On the basis of a comprehensive taxonomic study, we propose a novel species, Racemicystis persica sp. nov., for strain MSr11462T (=DSM 103165T=NCCB 100606T).
2015年，从伊朗波斯湾基什岛海滩采集的土壤样本中分离出一种新型黏细菌菌株MSr11462T。它表现出一般黏细菌的特征，如革兰氏阴性染色、杆状营养细胞、在固体表面滑行、微生物裂解活性、类子实体聚集体和类黏孢子结构。该菌株为嗜温菌、需氧菌，呈现化学异养营养模式。它对许多抗生素具有抗性，如庆大霉素、多黏菌素、夫西地酸和甲氧苄啶，全细胞水解产物的主要脂肪酸为异-C15 : 0、C16 : 0、异-C17 : 0、C18 : 1、异-C17 : 1 2-OH、C18 : 1 2-OH、异-C15 : 0 OAG（O-烷基甘油）和C16 : 1 OAG。16S rRNA基因序列与Racemicystis crocea菌株MSr9521T（GenBank登录号：KT591707）显示出最高的相似性（98.6 %）。基于16S rRNA基因序列和基质辅助激光解吸/电离飞行时间（MALDI-TOF）光谱数据的系统发育分析支持了多囊菌科Racemicystis属的一个新物种。DNA-DNA杂交显示新菌株与系统发育关系最密切的物种Racemicystis crocea MSr9521T之间只有约50 %的相似性。基于全面的分类学研究，我们为菌株MSr11462T（=DSM 103165T=NCCB 100606T）提出一个新物种，即波斯湾Racemicystis菌（Racemicystis persica sp. nov.）。
