Wong K R, Buckley J T
Department of Biochemistry and Microbiology, University of Victoria, BC, Canada.
Science. 1989 Nov 3;246(4930):654-6. doi: 10.1126/science.2814486.
Many Gram-negative bacteria export proteins to the exterior. Some of these proteins are first secreted into the periplasm and then cross the outer membrane in a separate step. The source of energy required for the translocation is unknown. Export of the extracellular protein proaerolysin from the periplasm through the outer membrane of Aeromonas salmonicida is inhibited by a proton ionophore and by low extracellular pH. One possible explanation of these results is that a proton gradient across the outer membrane is required for export.
许多革兰氏阴性菌会将蛋白质输出到细胞外。其中一些蛋白质首先被分泌到周质中，然后在单独的步骤中穿过外膜。转运所需的能量来源尚不清楚。嗜水气单胞菌周质中的细胞外蛋白气单胞菌素通过外膜的输出受到质子离子载体和低细胞外pH值的抑制。这些结果的一种可能解释是，外膜两侧的质子梯度是输出所必需的。
