Kong Shang, Ding Chen, Huang Lanlan, Bai Yan, Xiao Tiancun, Guo Jiao, Su Zhengquan
Key Research Center of Liver Regulation for Hyperlipidemia SATCM/Class III Laboratory of Metabolism SATCM, Guangdong TCM Key Laboratory for Metabolic Diseases, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Guangzhou Boxabio Technology Ltd, Guangzhou Science City, China.
Saudi J Biol Sci. 2017 Feb;24(2):251-255. doi: 10.1016/j.sjbs.2016.09.008. Epub 2016 Sep 10.
The objectives of this study were to explore the effect of COST (one thousand Da molecular weight chitosan oligosaccharide) on the differentiation of 3T3-L1 preadipocytes and to determine the mechanism of action. 3T3-L1 preadipocytes were used as the target cells, and the induction of the methods for the differentiation of 3T3-L1 preadipocytes was based on classic cocktails. The MTT assay was used to filtrate the concentration of COST. On the 6th day of induced-differentiation, the differentiation of 3T3-L1 cells was detected by Oil Red O staining. The expression of PPARγ and C/EBPα mRNA was determined using real-time fluorescence quantitative PCR (Q-PCR). COST inhibited 3T3-L1 preadipocyte differentiation in a dose-dependent manner and decreased lipid accumulation. At the molecular level, the expression of the transcription factors, PPARγ and C/EBPα, was reduced by COST during adipogenesis. These results indicate that COST effectively inhibited the differentiation of 3T3-L1 preadipocytes. The mechanism is related to the down-regulation expression of PPARγ and C/EBPα.
本研究的目的是探讨COST（一千道尔顿分子量的壳寡糖）对3T3-L1前脂肪细胞分化的影响，并确定其作用机制。以3T3-L1前脂肪细胞作为靶细胞，3T3-L1前脂肪细胞分化诱导方法基于经典组合。采用MTT法筛选COST的浓度。在诱导分化第6天，通过油红O染色检测3T3-L1细胞的分化情况。使用实时荧光定量PCR（Q-PCR）测定PPARγ和C/EBPα mRNA的表达。COST以剂量依赖性方式抑制3T3-L1前脂肪细胞分化并减少脂质积累。在分子水平上，COST在脂肪生成过程中降低了转录因子PPARγ和C/EBPα的表达。这些结果表明，COST有效抑制了3T3-L1前脂肪细胞的分化。其机制与PPARγ和C/EBPα的表达下调有关。
