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藜麦多糖的结构表征及其对3T3-L1脂肪细胞分化的抑制作用

Structural Characterization of Quinoa Polysaccharide and Its Inhibitory Effects on 3T3-L1 Adipocyte Differentiation.

作者信息

Teng Cong, Shi Zhenxing, Yao Yang, Ren Guixing

机构信息

Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.

Laboratory of Biomass and Green Technologies, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium.

出版信息

Foods. 2020 Oct 21;9(10):1511. doi: 10.3390/foods9101511.

DOI:10.3390/foods9101511
PMID:33096874
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7589720/
Abstract

Quinoa is a kind of nutritious food crop with anti-obesity activity, however, the mechanism is not unclear. In this study, we separated and purified bioactive polysaccharide from quinoa (denoted SQWP-2). The chemical structural was characterized and its effect on 3T3-L1 pre-adipocyte differentiation was evaluated. The molecular weight of SQWP-2 was found to be 7.49 × 10 Da, and the polysaccharide consisted of fructose and glucose. The Glc-(1→, Fru-(2→, →4)-Glcp-(1→, and →4,6)-Glcp-(1→ glycosidic linkages were identified in SQWP-2 through gas chromatography-mass spectrometry. Nuclear magnetic resonance confirmed the monosaccharide composition and glycosidic linkage content, and a suggestion of the structural formula is provided. In Western Blotting and RT-PCR assays, treatment with SQWP-2 significantly inhibited 3T3-L1 differentiation by suppressing PPARγ, C/EBPα, C/EBPβ, C/EBPδ, SREBP1C and AP2 expression. Quinoa polysaccharide isolated here could represent an anti-obesity agent once the structures and differentiation inhibition are definitively characterized.

摘要

藜麦是一种具有抗肥胖活性的营养粮食作物，然而，其作用机制尚不清楚。在本研究中，我们从藜麦中分离并纯化了生物活性多糖（命名为SQWP-2）。对其化学结构进行了表征，并评估了其对3T3-L1前脂肪细胞分化的影响。发现SQWP-2的分子量为7.49×10 Da，该多糖由果糖和葡萄糖组成。通过气相色谱-质谱法在SQWP-2中鉴定出Glc-(1→、Fru-(2→、→4)-Glcp-(1→和→4,6)-Glcp-(1→糖苷键。核磁共振证实了单糖组成和糖苷键含量，并给出了结构式的建议。在蛋白质免疫印迹和逆转录-聚合酶链反应分析中，用SQWP-2处理通过抑制PPARγ、C/EBPα、C/EBPβ、C/EBPδ、SREBP1C和AP2的表达显著抑制了3T3-L1的分化。一旦此处分离的藜麦多糖的结构和分化抑制作用得到明确表征，它可能成为一种抗肥胖剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/99928b0e64c9/foods-09-01511-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/2ef5d167940a/foods-09-01511-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/beea4a10968c/foods-09-01511-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/7f7c05669250/foods-09-01511-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/7e8377f67068/foods-09-01511-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/e13066bdb421/foods-09-01511-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/99928b0e64c9/foods-09-01511-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/2ef5d167940a/foods-09-01511-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/beea4a10968c/foods-09-01511-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/7f7c05669250/foods-09-01511-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/7e8377f67068/foods-09-01511-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/e13066bdb421/foods-09-01511-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/521b/7589720/99928b0e64c9/foods-09-01511-g006.jpg

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