Elshafie Hazem S, Racioppi Rocco, Bufo Sabino A, Camele Ippolito
School of Agricultural, Forestry, Food and Environmental Sciences, University of Basilicata, Viale Ateneo Lucano, 85100 Potenza, Italy.
Department of Science, University of Basilicata, Viale dell'Ateneo Lucano, 85100 Potenza, Italy.
Saudi J Biol Sci. 2017 Feb;24(2):295-301. doi: 10.1016/j.sjbs.2016.04.014. Epub 2016 May 2.
This research was carried out to study antibacterial activity of 4 strains of pv. () against G+ve and G-ve , haemolytic activity against the cell membrane of erythrocytes, the production of extracellular hydrolytic enzymes and finally, the pathogenicity against flesh blocks. Chemical structure of bioactive substances of the most bioactive strain (ICMP 11096) was established using gas chromatography-mass spectrometry (GC-MS). All the studied strains inhibited the growth of the two tested bacteria although some growing substrates negatively influenced the antimicrobial substance production. The same strains showed highly haemolytic activity and were able to produce 3 hydrolytic enzymes, i.e. chitinase, glucanase and protease. In pathogenicity assays, the considered strains resulted virulent for . . The GC-MS for compounds from ICMP 11096 were compatible with the structure of two bioactive fatty acids identified as methyl stearate and ethanol 2-butoxy phosphate with mass spectrum m/e 298 and 398, respectively.
本研究旨在研究4株（） pv. 对革兰氏阳性菌和革兰氏阴性菌的抗菌活性、对红细胞细胞膜的溶血活性、细胞外水解酶的产生，以及最终对肉块的致病性。使用气相色谱-质谱联用仪（GC-MS）确定了活性最强的菌株（ICMP 11096）生物活性物质的化学结构。所有研究菌株均抑制了两种受试细菌的生长，尽管一些生长底物对抗菌物质的产生有负面影响。相同的菌株表现出高度的溶血活性，并且能够产生3种水解酶，即几丁质酶、葡聚糖酶和蛋白酶。在致病性试验中，所考虑的菌株对……具有毒性。来自ICMP 11096的化合物的GC-MS与鉴定为硬脂酸甲酯和2-丁氧基磷酸乙醇酯的两种生物活性脂肪酸的结构相符，其质谱分别为m/e 298和398。
