Perin L, Martínez-Aguilar L, Paredes-Valdez G, Baldani J I, Estrada-de Los Santos P, Reis V M, Caballero-Mellado J
Embrapa Agrobiology, km 47, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil.
Universidade Federal Rural do Rio de Janeiro, km 45, BR 465, C.P. 74505, Seropédica, Rio de Janeiro, Brazil.
Int J Syst Evol Microbiol. 2006 Aug;56(Pt 8):1931-1937. doi: 10.1099/ijs.0.64362-0.
In a previous study, nitrogen-fixing isolates were recovered from the rhizosphere of maize and from surface-sterilized leaves of sugar cane cultivated in Rio de Janeiro, Brazil. On the basis of 16S rRNA gene sequence similarities, these isolates were identified as belonging to the genus Burkholderia, and whole-cell-protein profiles demonstrated that they are closely related to each other. In the present study, novel isolates were recovered from the roots of different sugar-cane varieties cultivated in diverse geographical regions of Brazil. Twenty-one nitrogen-fixing isolates were analysed using polyphasic taxonomy criteria, including DNA-DNA relatedness, 16S rRNA gene sequence similarities, fatty acid profiles, whole-cell-protein patterns and multilocus enzyme electrophoresis profiles, as well as morphological, physiological and biochemical characterization. The analysis confirmed that these isolates belong to a novel species within the genus Burkholderia, for which the name Burkholderia silvatlantica sp. nov. is proposed. The type strain, SRMrh-20(T) (=LMG 23149(T)=ATCC BAA-1244(T)), was isolated from the rhizosphere of maize var. Avantis A2345 cultivated in Seropédica, Rio de Janeiro.
在之前的一项研究中，从巴西里约热内卢种植的玉米根际以及甘蔗表面消毒叶片中分离出了固氮菌株。基于16S rRNA基因序列相似性，这些菌株被鉴定为伯克霍尔德氏菌属，全细胞蛋白图谱表明它们彼此密切相关。在本研究中，从巴西不同地理区域种植的不同甘蔗品种的根部分离出了新的菌株。使用多相分类标准对21株固氮菌株进行了分析，包括DNA-DNA相关性、16S rRNA基因序列相似性、脂肪酸谱、全细胞蛋白模式和多位点酶电泳图谱，以及形态、生理和生化特征。分析证实这些菌株属于伯克霍尔德氏菌属内的一个新物种，为此提出了伯克霍尔德氏菌新种（Burkholderia silvatlantica sp. nov.）的名称。模式菌株SRMrh-20(T)（=LMG 23149(T)=ATCC BAA-1244(T)）是从里约热内卢塞罗佩迪卡种植的玉米品种Avantis A2345的根际分离得到的。
