Camele Ippolito, Sadeek Sadeek A, Racioppi Rocco, Elshafie Hazem S
School of Agricultural, Forestry, Food and Environment al Sciences, University of Basilicata, Viale dell'Ateneo Lucano 10, 85100 Potenza, Italy.
Department of Chemistry, Faculty of Science, University of Zagazig, Zagazig 44519, Egypt.
Plants (Basel). 2023 Aug 3;12(15):2854. doi: 10.3390/plants12152854.
The genus includes important entomopathogenic and endophytic fungi; among them, is the most studied species. However, there is little knowledge regarding their antimicrobial activity. The current research has been conducted to evaluate the in vitro antagonistic activity of and the antimicrobial efficacy of its and metabolites against (Gram positive bacteria, G+ve), and (Gram negative bacteria, G-ve). In addition, solid-phase microextraction (SPME) was coupled with Gas Chromatography-Mass Spectrometry (GC/MS) to qualitatively measure the volatile organic compounds' (VOCs) metabolic profile of the most efficient studied isolate of . The obtained results showed that the isolate UniB2439-3 has a promising antibacterial effect against most of the studied target bacteria. An SPME-GC/MS analysis of VOCs revealed the presence of ethanol, butanal,2-methyl, 2,4-dimethyl-1-heptene, octane, 4-methyl and β-elemene as the dominant bioactive compounds. The results demonstrated that the efficient isolate of can be potentially used as a biocontrol agent against several bacteria, especially G+ve ones.
该属包括重要的昆虫病原真菌和内生真菌；其中，[具体物种名称]是研究最多的物种。然而，关于它们的抗菌活性知之甚少。目前已开展研究以评估[具体物种名称]的体外拮抗活性及其[具体提取物名称]和[具体提取物名称]代谢产物对金黄色葡萄球菌（革兰氏阳性菌，G+ve）、大肠杆菌和铜绿假单胞菌（革兰氏阴性菌，G-ve）的抗菌效果。此外，将固相微萃取（SPME）与气相色谱-质谱联用（GC/MS），以定性测定所研究的[具体物种名称]最有效分离株的挥发性有机化合物（VOCs）代谢谱。所得结果表明，分离株UniB2439-3对大多数所研究的目标细菌具有良好的抗菌效果。对VOCs的SPME-GC/MS分析显示，乙醇、丁醛、2-甲基丁醛、2,4-二甲基-1-庚烯、辛烷、4-甲基辛烷和β-榄香烯为主要生物活性化合物。结果表明，[具体物种名称]的有效分离株有可能用作针对多种细菌，尤其是革兰氏阳性菌的生物防治剂。
