Yunnan Provincial Observation and Research Station of Soil Degradation and Restoration for Cultivating Plateau Traditional Chinese Medicinal Plants, Yunnan Normal University, Kunming, 650500, Yunnan, China.
Wenshan Academy of Agricultural Sciences, Wenshan, 663000, Yunnan, China.
Curr Genet. 2024 Mar 30;70(1):4. doi: 10.1007/s00294-024-01288-4.
Panax notoginseng (Burkill) F.H. Chen, a valuable traditional Chinese medicine, faces significant yield and quality challenges stemming from root rot primarily caused by Fusarium solani. Burkholderia arboris PN-1, isolated from the rhizosphere soil of P. notoginseng, demonstrated a remarkable ability to inhibit the growth of F. solani. This study integrates phenotypic, phylogenetic, and genomic analyses to enhance our understanding of the biocontrol mechanisms employed by B. arboris PN-1. Phenotype analysis reveals that B. arboris PN-1 effectively suppresses P. notoginseng root rot both in vitro and in vivo. The genome of B. arboris PN-1 comprises three circular chromosomes (contig 1: 3,651,544 bp, contig 2: 1,355,460 bp, and contig 3: 3,471,056 bp), with a 66.81% GC content, housing 7,550 protein-coding genes. Notably, no plasmids were detected. Phylogenetic analysis places PN-1 in close relation to B. arboris AU14372, B. arboris LMG24066, and B. arboris MEC_B345. Average nucleotide identity (ANI) values confirm the PN-1 classification as B. arboris. Comparative analysis with seven other B. arboris strains identified 4,628 core genes in B. arboris PN-1. The pan-genome of B. arboris appears open but may approach closure. Whole-genome sequencing revealed 265 carbohydrate-active enzymes and identified 9 gene clusters encoding secondary metabolites. This comprehensive investigation enhances our understanding of B. arboris genomes, paving the way for their potential as effective biocontrol agents against fungal plant pathogens in the future.
三七（Burkill）F.H. Chen，一种有价值的传统中药，由于主要由尖孢镰刀菌引起的根腐病，面临着产量和质量的重大挑战。从三七根际土壤中分离出的鲍曼不动杆菌 PN-1 表现出显著抑制尖孢镰刀菌生长的能力。本研究整合表型、系统发育和基因组分析，以增强我们对鲍曼不动杆菌 PN-1 所采用的生物防治机制的理解。表型分析表明，鲍曼不动杆菌 PN-1 有效地抑制三七根腐病的发生，无论是在体外还是体内。鲍曼不动杆菌 PN-1 的基因组由三个环状染色体组成（contig 1：3,651,544 bp，contig 2：1,355,460 bp 和 contig 3：3,471,056 bp），GC 含量为 66.81%，包含 7,550 个编码蛋白质的基因。值得注意的是，没有检测到质粒。系统发育分析将 PN-1 与鲍曼不动杆菌 AU14372、鲍曼不动杆菌 LMG24066 和鲍曼不动杆菌 MEC_B345 紧密相关。平均核苷酸同一性（ANI）值证实 PN-1 被分类为鲍曼不动杆菌。与其他 7 株鲍曼不动杆菌菌株的比较分析确定了鲍曼不动杆菌 PN-1 中的 4,628 个核心基因。鲍曼不动杆菌的泛基因组似乎是开放的，但可能接近关闭。全基因组测序揭示了 265 种碳水化合物活性酶，并鉴定了 9 个编码次生代谢物的基因簇。这项全面的研究增强了我们对鲍曼不动杆菌基因组的理解，为它们未来作为有效生物防治剂对抗真菌植物病原体的潜力铺平了道路。
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