在评估抗菌药敏试验中，比较不同来源的抗生素药敏纸片在Quicolor琼脂培养基和穆勒-欣顿琼脂培养基上的情况。
A comparison of antibiotic disks from different sources on Quicolor and Mueller-Hinton agar media in evaluation of antibacterial susceptibility testing.
作者信息
Saffari Neda, Salmanzadeh-Ahrabi Siavosh, Abdi-Ali Ahya, Rezaei-Hemami Mohsen
机构信息
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran.
出版信息
Iran J Microbiol. 2016 Oct;8(5):307-311.
BACKGROUND AND OBJECTIVES
Antibacterial susceptibility testing of clinical bacterial isolates through disk diffusion method plays a major role in antibacterial treatment. One of the main factors affecting the result of these tests is the type, structure and quality of the disks. The main objective of this study was to compare the agreement of antibiotic disks originated from three companies on Quicolor and Mueller-Hinton agar.
MATERIALS AND METHODS
Quicolor and Mueller-Hinton agar media were used in disk diffusion method. Seventy clinical isolates from family (21 36 1 . and 12 ) were investigated in the study. After obtaining data, the results were interpreted as resistant, sensitive or intermediate. Kappa coefficient measured the agreement of two media. Coefficient of variation (CV) was also calculated for antibiotic disks.
RESULTS
The kappa agreement values for three types of antibiotic disks on Quicolor and Mueller-Hinton agar plates were good or excellent for all the examined antibiotics. CV values were also very satisfactory in the majority of cases.
CONCLUSION
Antibiotic disks from three manufacturers can successfully be used on both Quicolor and Mueller-Hinton agar plates.
背景与目的
通过纸片扩散法对临床分离细菌进行药敏试验在抗菌治疗中起着重要作用。影响这些试验结果的主要因素之一是纸片的类型、结构和质量。本研究的主要目的是比较来自三家公司的抗生素纸片在孔雀绿琼脂和穆勒-欣顿琼脂上的一致性。
材料与方法
在纸片扩散法中使用孔雀绿琼脂和穆勒-欣顿琼脂培养基。本研究调查了来自[具体菌属]的70株临床分离株（21株[细菌类型1]、36株[细菌类型2]和12株[细菌类型3]）。获取数据后，结果被解释为耐药、敏感或中介。kappa系数用于衡量两种培养基的一致性。还计算了抗生素纸片的变异系数（CV）。
结果
对于所有检测的抗生素，三种类型的抗生素纸片在孔雀绿琼脂平板和穆勒-欣顿琼脂平板上的kappa一致性值均为良好或优秀。在大多数情况下，CV值也非常令人满意。
结论
来自三个制造商的抗生素纸片均可成功用于孔雀绿琼脂平板和穆勒-欣顿琼脂平板。
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