Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Centre for Tissue Restoration and Reconstruction, Institute for Advanced Study and Division of Life Science , The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon 00852, Hong Kong.
Shenzhen Center for Disease Control and Prevention , Shenzhen 518055 , China.
ACS Nano. 2018 Sep 25;12(9):9549-9557. doi: 10.1021/acsnano.8b05270. Epub 2018 Aug 29.
Sensitive and accurate detection of highly contagious virus is urgently demanded for disease diagnosis and treatment. Herein, based on a multifunctional aggregation-induced emission luminogen (AIEgen), a dual-modality readout immunoassay platform for ultrasensitive detection of viruses has been successfully demonstrated. The platform is relied on virions immuno-bridged enzymatic hydrolysis of AIEgen, accompanying with the in situ formation of highly emissive AIE aggregates and shelling of silver on gold nanoparticles. As a result, robust turn-on fluorescence and naked-eye discernible plasmonic colorimetry composed dual-signal is achieved. By further taking advantage of effective immunomagnetic enrichment, EV71 virions, as an example, can be specifically detected with a limit of detection down to 1.4 copies/μL under fluorescence modality. Additionally, semiquantitative discerning of EV71 virions is realized in a broad range from 1.3 × 10 to 2.5 × 10 copies/μL with the naked eye. Most importantly, EV71 virions in 24 real clinical samples are successfully diagnosed with 100% accuracy. Comparing to the gold standard polymerase chain reaction (PCR) assay, our immunoassay platform do not need complicated sample pretreatment and expensive instruments. This dual-modality strategy builds a good capability for both colorimetry based convenient preliminary screening and fluorescence based accurate diagnosis of suspect infections in virus-stricken areas.
灵敏准确地检测高传染性病毒对于疾病的诊断和治疗至关重要。在此，我们基于多功能聚集诱导发射发光体（AIEgen），成功构建了一种用于超灵敏检测病毒的双模式读出免疫分析平台。该平台依赖于病毒免疫桥连的酶解 AIEgen，同时伴随着高发射 AIE 聚集物的原位形成和金纳米粒子上的银壳化。结果，实现了强开启的荧光和肉眼可辨别的等离子体比色双信号。通过进一步利用有效的免疫磁富集，EV71 病毒作为实例，可在荧光模式下以低至 1.4 拷贝/μL 的检测限进行特异性检测。此外，通过肉眼可在 1.3×10 到 2.5×10 拷贝/μL 的宽范围内实现对 EV71 病毒的半定量识别。最重要的是，我们成功地用该免疫分析平台对 24 个实际临床样本中的 EV71 病毒进行了诊断，准确率达到 100%。与金标准聚合酶链反应（PCR）检测相比，我们的免疫分析平台不需要复杂的样本预处理和昂贵的仪器。这种双模式策略为病毒感染地区的比色法便捷初步筛查和荧光法准确诊断提供了良好的能力。
