Kawano K, Kim Y I, Kaketani K, Kobayashi M
Department of Surgery I, Oita Medical College, Japan.
Transplantation. 1989 Nov;48(5):759-64. doi: 10.1097/00007890-198911000-00007.
The effect of pretreatment with cyclosporine on normothermic liver ischemia was studied in rats. The rats were divided into two groups. In group I, the animals were subjected to 60 min of warm liver ischemia. In group II, CsA (10 mg/kg, p.o.) was given daily for 4 days before induction of liver ischemia. The survival rates, serum biochemical parameters, and extent of necrosis (% liver necrosis), mitotic activities, and histology of the livers were investigated from days 1 to 7 following the release of hepatic vascular occlusion. CsA pretreatment significantly improved the survival of the animals (75.8%) in comparison with the control (42.5%). The highest levels of serum transaminases in group II had a tendency to be lower than in group I. The extent of liver necrosis reached a maximum in both groups one day postsurgery when the transaminases rose highest; however, the peak value of percentage of liver necrosis was significantly ameliorated by CsA treatment (27.1%) as compared with the control (48.3%). Furthermore, mitosis of hepatocytes in the group treated with CsA was substantially increased at day 3. Histological alterations of the livers coincided with the changes in other parameters. Our results demonstrate that CsA is capable of protecting the liver from ischemic insult. The mechanisms by which CsA exerts its beneficial effect on normothermic liver ischemia are discussed.
研究了环孢素预处理对大鼠常温肝脏缺血的影响。将大鼠分为两组。第一组动物接受60分钟的温性肝脏缺血。第二组在肝脏缺血诱导前4天每天口服环孢素（10mg/kg）。在解除肝血管阻塞后的第1至7天，研究动物的存活率、血清生化参数、坏死程度（肝坏死百分比）、有丝分裂活性以及肝脏组织学。与对照组（42.5%）相比，环孢素预处理显著提高了动物的存活率（75.8%）。第二组血清转氨酶的最高水平有低于第一组的趋势。两组在术后一天转氨酶升高最高时肝坏死程度均达到最大值；然而，与对照组（48.3%）相比，环孢素治疗显著改善了肝坏死百分比的峰值（27.1%）。此外，在第3天，环孢素治疗组肝细胞的有丝分裂显著增加。肝脏的组织学改变与其他参数的变化一致。我们的结果表明，环孢素能够保护肝脏免受缺血损伤。讨论了环孢素对常温肝脏缺血发挥有益作用的机制。
