Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Wuhan University, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
Talanta. 2017 Apr 1;165:188-193. doi: 10.1016/j.talanta.2016.12.051. Epub 2016 Dec 21.
Novel covalent organic framework-1 modified magnetic nanoparticles (M-COF-1) were prepared for extraction of paclitaxel from rat plasma samples. Paclitaxel is an important anti-cancer drug in clinical chemotherapy. COF-1 was immobilized on the surface of magnetic nanoparticles by bio-inspired polydopamine functionalization method because polydopamine can supply catechol groups for supporting the immobilization of COF-1. The formation of M-COF-1 has been confirmed by Fourier transform infrared spectroscopy and transmission electron microscopy. A M-COF-1-based extraction method was developed for selective extraction of paclitaxel in aqueous solutions. Extraction conditions were investigated, including sample pH value, acetonitrile content, extraction time and sample volume. By combining with HPLC, this method showed a good linear range of 0.1-200ng/mL with a low limit of detection of 0.02ng/mL. The method was also applied for the pretreatment of paclitaxel in rat plasma samples, which showed effective enrichment efficiency and good clean-up capacity. Recoveries were calculated to be 99.4-103.7% with relative standard deviations less than 2.3%.
新型共价有机框架-1 修饰的磁性纳米粒子（M-COF-1）被制备用于从大鼠血浆样品中提取紫杉醇。紫杉醇是临床化疗中一种重要的抗癌药物。COF-1 通过仿生聚多巴胺功能化方法固定在磁性纳米粒子的表面上，因为聚多巴胺可以提供儿茶酚基团来支持 COF-1 的固定。M-COF-1 的形成已经通过傅里叶变换红外光谱和透射电子显微镜得到了确认。建立了一种基于 M-COF-1 的萃取方法，用于在水溶液中选择性萃取紫杉醇。考察了萃取条件，包括样品 pH 值、乙腈含量、萃取时间和样品体积。该方法与 HPLC 结合，在 0.1-200ng/mL 范围内具有良好的线性关系，检测限低至 0.02ng/mL。该方法还应用于大鼠血浆样品中紫杉醇的预处理，显示出有效的富集效率和良好的净化能力。回收率计算为 99.4-103.7%，相对标准偏差小于 2.3%。
