College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Talanta. 2017 Apr 1;165:313-320. doi: 10.1016/j.talanta.2016.12.072. Epub 2016 Dec 25.
A surface-enhanced Raman scattering (SERS)-functionalized, gold nanoparticle (AuNPs)-immobilized paper strip was developed to realize label-free rapid detection of brassinosteroids (BRs) in plant tissues. The AuNPs paper strip was fabricated through utilizing poly(γ-glutamic acid) (γ-PGA) as the linker and concentrator for the directly immobilized AuNPs on paper, which can circumvent many drawbacks of the existing SERS substrates. An enhancement factor of 1.13×10, high reproducibility (relative standard deviation of 4.78%), highly sensitive detection limit with a correlation coefficient of 0.99 (1×10M 4-mercaptophenylboronic acid), and long-term stability for the responses of the SERS-functionalized AuNPs paper strip to Raman intensities were achieved by optimizing reaction conditions including the concentrations of γ-PGA and HAuCl/NaBH. Ultimately, this developed paper strip was applied to the determination of BRs in plant tissues, obtaining precisions with RSD values in a range of 3.0-13.8%. The SERS-functionalized AuNPs paper strip featuring label-free rapid analysis, and high sensitivity could serve as an excellent alternative for on-site rapid detections.
一种表面增强拉曼散射（SERS）功能化、金纳米粒子（AuNPs）固定化纸条被开发出来，以实现对植物组织中油菜素内酯（BRs）的无标记快速检测。AuNPs 纸条是通过利用聚（γ-谷氨酸）（γ-PGA）作为连接体和浓缩剂，将直接固定在纸条上的 AuNPs 固定在纸上，从而避免了现有 SERS 基底的许多缺点。通过优化包括γ-PGA 和 HAuCl/NaBH 浓度在内的反应条件，实现了 1.13×10 的增强因子、高重现性（相对标准偏差为 4.78%）、高灵敏度检测限，相关系数为 0.99（1×10M 4-巯基苯硼酸），以及 SERS 功能化 AuNPs 纸条对拉曼强度的响应的长期稳定性。最终，该开发的纸条被应用于植物组织中 BRs 的测定，获得了精度为 3.0-13.8%的 RSD 值。这种无标记快速分析、高灵敏度的 SERS 功能化 AuNPs 纸条可以作为现场快速检测的一种极好的替代品。
