Dumont Nicolas A, Rudnicki Michael A
Centre for Stem Cell Research, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, Canada, K1H 8L6.
Faculty of Medicine, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada.
Methods Mol Biol. 2017;1560:179-188. doi: 10.1007/978-1-4939-6788-9_12.
Muscle stem cells, named satellite cells, are quiescent in resting skeletal muscle. Following injury, satellite cells are activated and become proliferating myoblasts that either self-renew or differentiate. Several markers are used to identify the different myogenic populations, such as Pax7 (quiescent and activated satellite cells), MyoD (proliferating myoblasts), and myogenin (differentiating myoblasts). Immunodetection of these markers is a very useful tool to analyze myogenic cells and muscle regeneration. Here, we describe a method for immunodetection of satellite cells and their myogenic progeny in resting and regenerating skeletal muscles.
肌肉干细胞，即卫星细胞，在静止的骨骼肌中处于静止状态。受伤后，卫星细胞被激活并成为增殖的成肌细胞，这些成肌细胞要么自我更新，要么分化。几种标志物被用于识别不同的成肌细胞群体，如Pax7（静止和激活的卫星细胞）、MyoD（增殖的成肌细胞）和肌细胞生成素（分化的成肌细胞）。对这些标志物进行免疫检测是分析成肌细胞和肌肉再生的非常有用的工具。在这里，我们描述一种在静止和再生的骨骼肌中对卫星细胞及其成肌后代进行免疫检测的方法。
