Li Xian, Yan Yue, Lin Yuanzhe, Jiao Jian, Wang Da, Di Donghua, Zhang Ying, Jiang Tongying, Zhao Qinfu, Wang Siling
Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning Province 110016, PR China.
J Colloid Interface Sci. 2017 May 15;494:159-169. doi: 10.1016/j.jcis.2017.01.090. Epub 2017 Jan 25.
In this study, hollow mesoporous carbon nanoparticles (HMCN) and mesoporous carbon nanoparticles (MCN) were used as near-infrared region (NIR) nanomaterials and drug nanocarriers were prepared using different methods. A comparison between HMCN and MCN was performed with regard to the NIR-induced photothermal effect and drug loading efficiency. The results of NIR-induced photothermal effect test demonstrated that HMCN-COOH had a better photothermal conversion efficacy than MCN-COOH. Given the prominent photothermal effect of HMCN-COOH in vitro, the chemotherapeutic drug DOX was chosen as a model drug to further evaluate the drug loading efficiencies and NIR-triggered drug release behaviors of the nanocarriers. The drug loading efficiency of DOX/HMCN-COOH was found to be up to 76.9%, which was higher than that of DOX/MCN-COOH. In addition, the use of an 808nm NIR laser markedly increased the release of DOX from both carbon carriers in pH 5.0 PBS and pH 7.4 PBS. Cellular photothermal tests involving A549 cells demonstrated that HMCN-COOH had a much higher photothermal efficacy than MCN-COOH. Cell viability experiments and flow cytometry were performed to evaluate the therapeutic effect of DOX/HMCN-COOH and the results obtained demonstrated that DOX/HMCN-COOH had a synergistic therapeutic effect in cancer treatment involving a combination of chemotherapy and photothermal therapy.
在本研究中，中空介孔碳纳米颗粒（HMCN）和介孔碳纳米颗粒（MCN）被用作近红外区域（NIR）的纳米材料，并采用不同方法制备了药物纳米载体。就近红外诱导的光热效应和药物负载效率对HMCN和MCN进行了比较。近红外诱导光热效应测试结果表明，HMCN-COOH比MCN-COOH具有更好的光热转换效率。鉴于HMCN-COOH在体外具有显著的光热效应，选择化疗药物阿霉素（DOX）作为模型药物，进一步评估纳米载体的药物负载效率和近红外触发的药物释放行为。发现DOX/HMCN-COOH的药物负载效率高达76.9%，高于DOX/MCN-COOH。此外，使用808nm近红外激光显著增加了DOX在pH 5.0 PBS和pH 7.4 PBS中从两种碳载体的释放。涉及A549细胞的细胞光热测试表明，HMCN-COOH比MCN-COOH具有更高的光热效率。进行了细胞活力实验和流式细胞术以评估DOX/HMCN-COOH的治疗效果，所得结果表明，DOX/HMCN-COOH在涉及化疗和光热疗法联合的癌症治疗中具有协同治疗效果。
