Costa Arantes Diego Antonio, Gonçalves Andréia Souza, Jham Bruno Correia, Duarte Eliza Carla Barroso, de Paula Élbio Candido, de Paula Henrique Moura, Mendonça Elismauro Francisco, Batista Aline Carvalho
Department of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Goiânia, Brazil.
College of Dental Medicine-Illinois, Midwestern University, Downers Grove, IL, USA.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2017 Jun;123(6):e188-e196. doi: 10.1016/j.oooo.2016.12.002. Epub 2016 Dec 14.
The aim of this study was to investigate the expression of human leukocyte antigens (HLAs) G and E and programmed death-ligand 1 (PD-L1) in oral osteosarcoma (OO) (n = 13). The relationship between the expression of these molecules and histologic grading and metastasis was also evaluated.
HLA-G, HLA-E, and PD-L1 were identified by immunohistochemistry. Samples of normal bone tissue (n = 6) were used as controls. The sections were evaluated using a semiquantitative scoring system with an immunoreactive score, where a score of 0 was considered absent, ≤2 was low, and >2 was high expression.
We identified high expression of HLA-G, HLA-E, and PD-L1 by malignant osteoblastic cells in 69.2% of OO cases, which was statistically higher than that in controls (P < .05). Overexpression of these proteins was identified in 8 of 11 samples of high-grade and 1 of 2 samples of low-grade OO. Additionally, 66.6% of patients with metastases (n = 4) and 71.4% of patients without metastases (n = 5) had high expression of HLA-G, HLA-E, and PD-L1 in tumor samples (P > .05).
OO had high expression of HLA-G, HLA-E, and PD-L1 irrespective of clinicopathologic parameters, including histologic grading and metastasis.
本研究旨在调查人白细胞抗原（HLA）G和E以及程序性死亡配体1（PD-L1）在口腔骨肉瘤（OO，n = 13）中的表达情况。还评估了这些分子的表达与组织学分级和转移之间的关系。
通过免疫组织化学鉴定HLA-G、HLA-E和PD-L1。正常骨组织样本（n = 6）用作对照。使用具有免疫反应评分的半定量评分系统评估切片，其中0分为无表达，≤2分为低表达，>2分为高表达。
我们在69.2%的OO病例中发现恶性成骨细胞高表达HLA-G、HLA-E和PD-L1，这在统计学上高于对照组（P <.05）。在11例高级别OO样本中的8例和2例低级别OO样本中的1例中鉴定出这些蛋白的过表达。此外，在有转移的患者（n = 4）中有66.6%以及在无转移的患者（n = 5）中有71.4%在肿瘤样本中高表达HLA-G、HLA-E和PD-L1（P >.05）。
无论包括组织学分级和转移在内的临床病理参数如何，OO均高表达HLA-G、HLA-E和PD-L1。
