Kobayashi Naoya, Arai Ryoichi
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan; Department of Bioscience and Textile Technology, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan.
Department of Applied Biology, Faculty of Textile Science & Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan; Department of Bioscience and Textile Technology, Interdisciplinary Graduate School of Science and Technology, Shinshu University, 3-15-1 Tokida, Ueda, Nagano 386-8567, Japan; Department of Supramolecular Complexes, Research Center for Fungal & Microbial Dynamism, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399-4598, Japan; Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting Edge Research, Shinshu University, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan; Division of Structural and Synthetic Biology, RIKEN Center for Life Science Technologies, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan.
Curr Opin Biotechnol. 2017 Aug;46:57-65. doi: 10.1016/j.copbio.2017.01.001. Epub 2017 Feb 1.
The central goal of nanobiotechnology is to design and construct novel biomaterials of nanometer sizes. In this short review, we describe recent progress of several approaches for designing and creating artificial self-assembling protein complexes and primarily focus on the following biotechnological strategies for using artificial and fusion proteins as nanoscale building blocks: fusion proteins designed for symmetrical self-assembly; three-dimensional domain-swapped oligomers; self-assembling designed coiled-coil peptide modules; metal-directed self-assembling engineered proteins; computationally designed self-assembling de novo proteins; and self-assembling protein nanobuilding blocks (PN-Blocks) using an intermolecularly folded dimeric de novo protein. These state-of-the-art nanobiotechnologies for designing supramolecular protein complexes will facilitate the development of novel functional nanobiomaterials.
纳米生物技术的核心目标是设计和构建纳米尺寸的新型生物材料。在这篇简短的综述中，我们描述了几种设计和创建人工自组装蛋白复合物方法的最新进展，并主要关注以下将人工蛋白和融合蛋白用作纳米级构建模块的生物技术策略：为对称自组装设计的融合蛋白；三维结构域交换寡聚体；自组装设计的卷曲螺旋肽模块；金属导向自组装工程蛋白；通过计算设计的自组装全新蛋白；以及使用分子间折叠的二聚体全新蛋白的自组装蛋白纳米构建模块（PN-Blocks）。这些用于设计超分子蛋白复合物的前沿纳米生物技术将推动新型功能性纳米生物材料的发展。
