Peila Chiara, Coscia Alessandra, Bertino Enrico, Li Volti Giovanni, Galvano Fabio, Visser Gerard H A, Gazzolo Diego
a Neonatology Unit, Department of Public Health and Pediatrics , University of Turin , Turin , Italy.
b Department of Biological Chemistry, Medical Chemistry and Molecular Biology , University of Catania , Catania , Italy.
J Matern Fetal Neonatal Med. 2018 Feb;31(4):513-517. doi: 10.1080/14767058.2017.1291618. Epub 2017 Feb 28.
Donor milk (DM) represents an important nutrition source for high-risk newborns. Holder pasteurization (HoP) is the most recommended procedure for DM treatment, providing a good compromise between microbiological safety and biological quality. HoP was previously shown to affect DM cytokines, growth factors and hormones levels, whilst no data concerning the possible effects of HoP on neurobiomarkers (NB) are available. Therefore, our study investigated whether the concentration in DM of a well-known NB involved in brain development/damage, namely S100B, changes due to HoP.
We conducted a pretest-test study in 11 mothers, whose DM samples were sub-divided into two parts: the first was immediately frozen (-80 °C); the second was pasteurized with Holder method before freezing. S100B DM levels were measured using a commercially available immunoluminometric assay.
S100B protein was detected in all milk samples. Results showed significant differences between groups (p < 0.05) in S100B levels after HoP.
Our data provide evidence that S100B is present in preterm milk as well as in term milk during maturation degree. Moreover, the results confirm the susceptibility of this neurotrophic factor to pasteurization stresses and the need to develop new storage techniques to preserve the biological quality of human milk.
捐赠母乳（DM）是高危新生儿的重要营养来源。间歇式巴氏杀菌法（HoP）是DM处理最推荐的方法，能在微生物安全性和生物质量之间取得良好平衡。先前研究表明HoP会影响DM中细胞因子、生长因子和激素水平，但尚无关于HoP对神经生物标志物（NB）可能影响的数据。因此，我们的研究调查了一种参与大脑发育/损伤的知名NB即S100B在DM中的浓度是否会因HoP而改变。
我们对11位母亲进行了一项预测试-测试研究，其DM样本被分成两部分：第一部分立即冷冻（-80°C）；第二部分在冷冻前用间歇式巴氏杀菌法进行巴氏杀菌。使用市售免疫发光分析法测量S100B在DM中的水平。
在所有母乳样本中均检测到S100B蛋白。结果显示HoP后两组间S100B水平存在显著差异（p<0.05）。
我们的数据表明，S100B存在于早产母乳以及足月母乳成熟阶段。此外，结果证实了这种神经营养因子对巴氏杀菌应激的敏感性，以及开发新的储存技术以保持人乳生物质量的必要性。
