Bulle Saradamma, Reddy Vaddi Damodara, Padmavathi Pannuru, Maturu Paramahamsa, Puvvada Pavan Kumar, Nallanchakravarthula Varadacharyulu
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur - 515 003, AP, India.
Oil Technological Research Institute, Jawaharlal Nehru Technological University, Anantapur - 515 001, AP, India.
J Clin Biochem Nutr. 2017 Jan;60(1):63-69. doi: 10.3164/jcbn.16-16. Epub 2016 Oct 5.
The present study aimed to understand the association between erythrocyte membrane alterations and hemolysis in chronic alcoholics. Study was conducted on human male volunteers aged between 35-45 years with a drinking history of 8-10 years. Results showed that plasma marker enzymes AST, ALT, ALP and γGT were increased in alcoholic subjects. Plasma and erythrocyte membrane lipid peroxidation, erythrocyte lysate nitric oxide (NOx) levels were also increased significantly in alcoholics. Furthermore, erythrocyte membrane protein carbonyls, total cholesterol, phospholipid and cholesterol/phospholipid (C/P) ratio were increased in alcoholics. SDS-PAGE analysis of erythrocyte membrane proteins revealed that increased density of band 3, protein 4.2, 4.9, actin and glycophorins, whereas glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and glycophorin A showed slight increase, however, decreased ankyrin with no change in spectrins (α and β) and protein 4.1 densities were observed in alcoholics. Moreover, alcoholics red blood cells showed altered morphology with decreased resistance to osmotic hemolysis. Increased hemolysis showed strong positive association with lipid peroxidation ( = 0.703, <0.05), protein carbonyls ( = 0.754, <0.05), lysate NOx ( = 0.654, <0.05) and weak association with C/P ratio ( = 0.240, <0.05). Bottom line, increased lipid and protein oxidation, altered membrane C/P ratio and membrane cytoskeletal protein profile might be responsible for the increased hemolysis in alcoholics.
本研究旨在了解慢性酒精中毒患者红细胞膜改变与溶血之间的关联。研究对象为年龄在35 - 45岁之间、饮酒史为8 - 10年的男性志愿者。结果显示，酒精中毒受试者的血浆标记酶AST、ALT、ALP和γGT升高。酒精中毒者的血浆和红细胞膜脂质过氧化、红细胞裂解液一氧化氮（NOx）水平也显著升高。此外，酒精中毒者的红细胞膜蛋白羰基、总胆固醇、磷脂以及胆固醇/磷脂（C/P）比值升高。红细胞膜蛋白的SDS - PAGE分析显示，带3、蛋白4.2、4.9、肌动蛋白和血型糖蛋白的条带密度增加，而甘油醛 - 3 - 磷酸脱氢酶（GAPDH）和血型糖蛋白A略有增加，然而，酒精中毒者中锚蛋白减少，血影蛋白（α和β）和蛋白4.1密度无变化。此外，酒精中毒者的红细胞形态改变，对渗透性溶血的抵抗力降低。溶血增加与脂质过氧化（r = 0.703，P <0.05）、蛋白羰基（r = 0.754，P <0.05）、裂解液NOx（r = 0.654，P <0.05）呈强正相关，与C/P比值呈弱相关（r = 0.240，P <0.05）。总之，脂质和蛋白质氧化增加、膜C/P比值改变以及膜细胞骨架蛋白谱改变可能是酒精中毒者溶血增加的原因。
