不明原因复发性自然流产女性中出现的自然杀伤细胞减少，且药物治疗后进一步降低。
Declined Natural Killer Cells Emerging in Women with Unexplained Recurrent Spontaneous Abortion and Further Reducing after Medical Therapy.
作者信息
Lin Xianghua, Lin Dijin, Liu Jiao, Bao Yunwen, Luo Zhaofan, Fang Junyue, Wang Ying, Zhao Mingpeng, Duan Chaohui
出版信息
Clin Lab. 2016 Nov 1;62(11):2241-2247. doi: 10.7754/Clin.Lab.2016.160603.
BACKGROUND
As focus grows on reproduction, the issue of Recurrent Spontaneous Abortion (RSA), especially for unexplained reasons (URSA), is grabbing more and more attention in gynecological immunology. We investigated the changes of peripheral lymphocyte subsets focusing on whether they had some relationship with development of URSA.
METHODS
The percentage and absolute count of lymphocyte subsets (T cells, Th cells, Ts cells, B cells, NK cells) were simultaneously evaluated by flow cytometry in URSA patients (n = 48) and healthy controls (HC, n = 22).
RESULTS
Significantly lower percentage and absolute counts of NKT cells and NK cells were observed in URSA compared to the HC. After medical therapy, an obviously increase was shown in the percentage of both T cells and B cells, whereas it presented a reduction in the percentage of NK cells.
CONCLUSIONS
The flow cytometry test in T, B, NK cells is a method available to identify URSA patients from healthy women and to provide reference guides for clinical therapy.
背景
随着对生殖问题的关注度不断提高，复发性自然流产（RSA）问题，尤其是不明原因的复发性自然流产（URSA），在妇科免疫学中越来越受到关注。我们以外周血淋巴细胞亚群的变化为研究重点，探讨其与URSA发生发展是否存在某种关联。
方法
采用流式细胞术同时检测48例URSA患者和22例健康对照者外周血淋巴细胞亚群（T细胞、Th细胞、Ts细胞、B细胞、NK细胞）的百分比及绝对计数。
结果
与健康对照者相比，URSA患者NKT细胞和NK细胞的百分比及绝对计数显著降低。药物治疗后，T细胞和B细胞的百分比明显升高，而NK细胞的百分比则降低。
结论
对T、B、NK细胞进行流式细胞术检测，是一种区分URSA患者与健康女性的可行方法，可为临床治疗提供参考依据。
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