LuIs Henrique Soares, Luis Luis Soares, Bernardo Mário
Lisbon Dental Medicine School, University of Lisbon, Leiria, Portugal.
Health School, Polytechnique Institute, Leiria, Portugal.
Indian J Dent Res. 2016 Nov-Dec;27(6):648-651. doi: 10.4103/0970-9290.199602.
Mouthrinses are used, by many of our patients, as a complement to daily dental hygiene routine. The use of a toothbrush and an interproximal cleaning method may not be enough to control dental plaque. Essential oils and delmopinol mouth rinses are effective for the prevention of dental caries and gingivitis. To study the effect of an essential oil and a delmopinol mouth rinse on dental plaque bacteria, an in vitro study was developed.
The objective of this study was to determine the antibacterial activity of an essential oil and a delmopinol mouth rinse on Streptococcus mutans, Lactobacilli, and aerobic and anaerobic dental plaque nonspecific bacteria.
Samples of human dental plaque were collected from consenting participants and bacteria isolated. Disk-diffusion tests were performed to obtain the minimum concentration of the mouth rinses necessary to inhibit bacterial growth. The ability of the commercial mouth rinses to inhibit bacterial growth was studied in comparison to a positive control (0.2% chlorhexidine) and a negative laboratorial control (sterilized water).
The minimum inhibitory concentration was found to be inferior to the commercial essential oils and delmopinol mouth rinses concentrations. Delmopinol and essential oils have significant antibacterial properties shown in vitro only for aerobic bacteria, and for S. mutans, Lactobacillus, and anaerobic bacteria, the results were not statistically significant.
Essential oils and chlorhexidine are statistically similar and better than delmopinol for aerobic bacteria growth inhibition. For the other bacteria, essential oils and delmopinol are not statistically promising. Results show that essential oils only may help patients to maintain good oral health as a complement to daily brushing and interproximal cleaning.
我们的许多患者使用漱口水作为日常口腔卫生护理的补充。仅使用牙刷和牙间隙清洁方法可能不足以控制牙菌斑。精油漱口水和地莫匹醇漱口水对预防龋齿和牙龈炎有效。为了研究精油漱口水和地莫匹醇漱口水对牙菌斑细菌的影响,开展了一项体外研究。
本研究的目的是确定精油漱口水和地莫匹醇漱口水对变形链球菌、乳酸杆菌以及需氧和厌氧牙菌斑非特异性细菌的抗菌活性。
从同意参与的受试者中采集人类牙菌斑样本并分离细菌。进行纸片扩散试验以获得抑制细菌生长所需的漱口水最低浓度。将市售漱口水与阳性对照(0.2%氯己定)和阴性实验室对照(灭菌水)进行比较,研究其抑制细菌生长的能力。
发现最低抑菌浓度低于市售精油漱口水和地莫匹醇漱口水的浓度。地莫匹醇和精油仅对需氧菌显示出显著的体外抗菌特性,而对于变形链球菌、乳酸杆菌和厌氧菌,结果无统计学意义。
对于抑制需氧菌生长,精油和氯己定在统计学上相似且优于地莫匹醇。对于其他细菌,精油和地莫匹醇在统计学上没有前景。结果表明,精油仅作为日常刷牙和牙间隙清洁的补充,可能有助于患者保持良好的口腔健康。