Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital Affiliated with Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics, and Division of Exercise Physiology, West Virginia University, Morgantown, West Virginia, USA.
Stem Cells Transl Med. 2017 Jan;6(1):306-315. doi: 10.5966/sctm.2015-0426. Epub 2016 Aug 8.
Finding appropriate seed cells for bone tissue engineering remains a significant challenge. Considering that skin is the largest organ, we hypothesized that human bone morphogenetic protein receptor type IB (BmprIB)+ dermal cells could have enhanced osteogenic capacity in the healing of critical-sized calvarial defects in an immunodeficient mouse model. In this study, immunohistochemical staining revealed that BmprIB was expressed throughout reticular dermal cells; the positive expression rate of BmprIB was 3.5% ± 0.4% in freshly separated dermal cells, by flow cytometry. Furthermore, in vitro osteogenic capacity of BmprIB+ cells was confirmed by osteogenic-related staining and marker gene expression compared with unsorted dermal cells. In vivo osteogenic capacity was demonstrated by implantation of human BmprIB+ cell/coral constructs in the treatment of 4-mm diameter calvarial defects in an immunodeficient mouse model compared with implantation of unsorted cell/coral constructs and coral scaffold alone. These results indicate that the selective cell population BmprIB from human dermis is a promising osteogenic progenitor cell that can be a large-quantity and high-quality cell source for bone tissue engineering and regeneration. Stem Cells Translational Medicine 2017;6:306-315.
寻找合适的种子细胞用于骨组织工程仍然是一个重大挑战。考虑到皮肤是最大的器官，我们假设人骨形态发生蛋白受体 1B（BmprIB）+真皮细胞在免疫缺陷小鼠模型中修复临界尺寸颅骨缺损时可能具有增强的成骨能力。在这项研究中，免疫组织化学染色显示 BmprIB 表达于网状真皮细胞中；通过流式细胞术，新鲜分离的真皮细胞中 BmprIB 的阳性表达率为 3.5%±0.4%。此外，与未分选的真皮细胞相比，通过成骨相关染色和标记基因表达证实了 BmprIB+细胞的体外成骨能力。通过将人 BmprIB+细胞/珊瑚构建体植入免疫缺陷小鼠模型中 4mm 直径颅骨缺损的治疗中，与植入未分选细胞/珊瑚构建体和珊瑚支架单独植入相比，证明了体内成骨能力。这些结果表明，从人真皮中选择性细胞群体 BmprIB 是一种有前途的成骨祖细胞，可为骨组织工程和再生提供大量高质量的细胞来源。干细胞转化医学 2017;6:306-315。
