基本原理：从单个细胞中组装 BCR。
BASIC: BCR assembly from single cells.
机构信息
Toyota Technological Institute at Chicago, Chicago, IL, USA
Committee on Immunology, The Knapp Center of Lupus and Immunology Research, The University of Chicago, Chicago, IL 60637, USA.
出版信息
Bioinformatics. 2017 Feb 1;33(3):425-427. doi: 10.1093/bioinformatics/btw631.
MOTIVATION
The B-cell receptor enables individual B cells to identify diverse antigens, including bacterial and viral proteins. While advances in RNA-sequencing (RNA-seq) have enabled high throughput profiling of transcript expression in single cells, the unique task of assembling the full-length heavy and light chain sequences from single cell RNA-seq (scRNA-seq) in B cells has been largely unstudied.
RESULTS
We developed a new software tool, BASIC, which allows investigators to use scRNA-seq for assembling BCR sequences at single-cell resolution. To demonstrate the utility of our software, we subjected nearly 200 single human B cells to scRNA-seq, assembled the full-length heavy and the light chains, and experimentally confirmed these results by using single-cell primer-based nested PCRs and Sanger sequencing.
AVAILABILITY AND IMPLEMENTATION
http://ttic.uchicago.edu/∼aakhan/BASIC
CONTACT
SUPPLEMENTARY INFORMATION
Supplementary data are available at Bioinformatics online.
动机
B 细胞受体使单个 B 细胞能够识别多种抗原，包括细菌和病毒蛋白。尽管 RNA 测序（RNA-seq）的进步使单细胞中转录物表达的高通量分析成为可能，但从单细胞 RNA-seq（scRNA-seq）中组装完整的重链和轻链序列的独特任务在很大程度上仍未得到研究。
结果
我们开发了一种新的软件工具 BASIC，它允许研究人员使用 scRNA-seq 以单细胞分辨率组装 BCR 序列。为了证明我们软件的实用性，我们对近 200 个人类 B 细胞进行了 scRNA-seq 处理，组装了全长重链和轻链，并通过使用单细胞基于引物的嵌套 PCR 和 Sanger 测序实验证实了这些结果。
可用性和实现
http://ttic.uchicago.edu/∼aakhan/BASIC
联系方式
补充信息
补充数据可在 Bioinformatics 在线获取。
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