Burke W J, Mattammal M B, Marshall G L, Chung H
Department of Neurology, Veterans Administration Medical Center, St. Louis, Missouri.
Anal Biochem. 1989 Jul;180(1):79-84. doi: 10.1016/0003-2697(89)90090-0.
The monoamine oxidase A metabolite of noradrenaline, 3,4-dihydroxyphenylglycolaldehyde, is the precursor of 3,4-dihydroxymandelic acid, and 3,4-dihydroxyphenylglycol, metabolites of noradrenaline. Owing to difficulties in purifying this aldehyde, it has not been previously characterized or identified in biological sources. This paper describes an enzymatic synthesis, purification, and characterization of 3,4-dihydroxyphenylglycolaldehyde. The aldehyde metabolite is identified in postmortem human brain using high-performance liquid chromatography and electrochemical detection. We estimate the concentration in human hippocampus to be 0.164 +/- 0.05 nmol/g. The importance of this aldehyde metabolite of noradrenaline is discussed.
去甲肾上腺素的单胺氧化酶A代谢产物3,4-二羟基苯乙醇醛，是去甲肾上腺素的代谢产物3,4-二羟基扁桃酸和3,4-二羟基苯乙二醇的前体。由于纯化这种醛存在困难，它以前尚未在生物来源中得到表征或鉴定。本文描述了3,4-二羟基苯乙醇醛的酶促合成、纯化及表征。使用高效液相色谱和电化学检测在人死后大脑中鉴定出了这种醛代谢产物。我们估计人脑海马体中的浓度为0.164±0.05 nmol/g。文中讨论了去甲肾上腺素的这种醛代谢产物的重要性。
