Burke W J, Schmitt C A, Gillespie K N, Li S W
Department of Neurology, Saint Louis University Medical School, MO, USA.
Brain Res. 1996 May 25;722(1-2):232-5. doi: 10.1016/0006-8993(96)00129-1.
3,4-Dihydroxyphenylglycolaldehyde (DOPEGAL) is the monoamine oxidase A (MAO-A) metabolite of norepinephrine (NE) and epinephrine (Epi). Oxidative metabolites of amines are predicted toxins. In this study we determine the toxicity of DOPEGAL, its tautomer 2',3,4-trihydroxyacetophenone (THAP) as well as NE, Epi and their oxidative and methylated metabolites in cultures of differentiated PC-12 cells. At 59.5 microM DOPEGAL, THAP and Epi, but not NE or other NE or Epi metabolites decreased PC-12 cells by 43.8%, 26.7% and 16.8% respectively. DOPEGAL toxicity was concentration and time dependent. Possible implications for degenerative diseases are discussed.
3,4-二羟基苯乙醇醛（DOPEGAL）是去甲肾上腺素（NE）和肾上腺素（Epi）的单胺氧化酶A（MAO-A）代谢产物。胺类的氧化代谢产物被认为是毒素。在本研究中，我们测定了DOPEGAL、其互变异构体2',3,4-三羟基苯乙酮（THAP）以及NE、Epi及其氧化和甲基化代谢产物在分化的PC-12细胞培养物中的毒性。在59.5微摩尔的DOPEGAL、THAP和Epi作用下，PC-12细胞分别减少了43.8%、26.7%和16.8%，而NE或其他NE或Epi代谢产物则没有这种作用。DOPEGAL的毒性具有浓度和时间依赖性。文中讨论了其对退行性疾病可能产生的影响。
