Burke W J, Chung H D, Li S W
Department of Neurology, Veterans Affairs Medical Center and Saint Louis University Medical School, St. Louis, Missouri 63110, USA.
Anal Biochem. 1999 Aug 15;273(1):111-6. doi: 10.1006/abio.1999.4196.
We recently described the chemical synthesis of 3, 4-dihydroxyphenylacetaldehyde and 3,4-dihydroxyphenylglycolaldehyde, the monamine oxidase metabolites of dopamine and noradrenaline, respectively. We demonstrated the neurotoxicity of these compounds. Catecholamine nerve cells which synthesize these aldehydes die in degenerative brain diseases, such as Parkinson's and Alzheimer's. Here we describe a sensitive method for separating these catecholaldehydes from catecholamines and their other oxidative and methylated metabolites by microcolumn high-performance liquid chromatography with electrochemical detection. We then quantitate catecholamines and their major metabolites in human brain, plasma, and urine. The method can be used to determine the role of these catecholaldehydes in human disease.
我们最近描述了3,4-二羟基苯乙醛和3,4-二羟基苯乙醇醛的化学合成，它们分别是多巴胺和去甲肾上腺素的单胺氧化酶代谢产物。我们证明了这些化合物具有神经毒性。合成这些醛类的儿茶酚胺神经细胞会在帕金森氏症和阿尔茨海默氏症等退行性脑部疾病中死亡。在此，我们描述了一种灵敏的方法，通过带有电化学检测的微柱高效液相色谱法，将这些儿茶酚醛与儿茶酚胺及其其他氧化和甲基化代谢产物分离。然后我们对人脑、血浆和尿液中的儿茶酚胺及其主要代谢产物进行定量分析。该方法可用于确定这些儿茶酚醛在人类疾病中的作用。
