Surtees R, Hyland K
Department of Child Health, Institute of Child Health, London, United Kingdom.
Anal Biochem. 1989 Sep;181(2):331-5. doi: 10.1016/0003-2697(89)90252-2.
A method for the measurement of S-adenosylmethionine in cerebrospinal fluid and brain using high-performance liquid chromatography with electrochemical detection is described. The method is based upon the catechol O-methyltransferase-catalyzed methylation of dihydroxybenzylamine to its 3- and 4-methoxy derivatives and measurement of the latter. Detection limits are 0.1 pmol for 3- and 4-methoxyhydroxybenzylamine, recovery is 100%, and variability is less than 8%. HPLC measurement of 3- and 4-methoxyhydroxybenzylamine is linear up to at least 100 pmol injected. The use of a nonbiological catechol in the assay minimizes interference from endogenous and exogenous catechols and the wide limits of linearity allows the assay of small volumes of cerebrospinal fluid or brain extract without further concentration or dilution.
描述了一种使用高效液相色谱-电化学检测法测量脑脊液和脑组织中S-腺苷甲硫氨酸的方法。该方法基于儿茶酚O-甲基转移酶催化二羟基苄胺甲基化为其3-和4-甲氧基衍生物并对后者进行测量。3-和4-甲氧基羟基苄胺的检测限为0.1皮摩尔，回收率为100%，变异率小于8%。注入高达至少100皮摩尔的3-和4-甲氧基羟基苄胺时，其高效液相色谱测量呈线性。该测定中使用非生物儿茶酚可最大限度减少内源性和外源性儿茶酚的干扰，宽线性范围允许在不进一步浓缩或稀释的情况下测定少量脑脊液或脑提取物。
