Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA.
Department of Internal Medicine, Division of Gastroenterology, Rush University Medical Center, Chicago, IL 60612, USA.
Food Funct. 2017 Mar 22;8(3):1166-1173. doi: 10.1039/c6fo01532h.
Impaired gut barrier function plays an important role in the development of many diseases such as obesity, inflammatory bowel disease, and in HIV infection. Dietary fibres have been shown to improve intestinal barrier function through their fermentation products, short chain fatty acids (SCFAs), and the effects of individual SCFAs have been studied. Here, different SCFA mixtures representing possible compositions from fibre fermentation products were studied for protective and reparative effects on intestinal barrier function. The effect of fermentation products from four dietary fibres, i.e. resistant starch, fructooligosaccharides, and sorghum and corn arabinoxylan (varying in their branched structure) on barrier function was positively correlated with their SCFA concentration. Pure SCFA mixtures of various concentrations and compositions were tested using a Caco-2 cell model. SCFAs at a moderate concentration (40-80 mM) improved barrier function without causing damage to the monolayer. In a 40 mM SCFA mixture, the butyrate proportion at 20% and 50% showed both a protective and a reparative effect on the monolayer to disrupting agents (LPS/TNF-α) applied simultaneously or prior to the SCFA mixtures. Relating this result to dietary fibre selection, slow fermenting fibres that deliver appropriate concentrations of SCFAs to the epithelium with a high proportion of butyrate may improve barrier function.
肠道屏障功能障碍在许多疾病的发展中起着重要作用，如肥胖、炎症性肠病和 HIV 感染。膳食纤维已被证明通过其发酵产物、短链脂肪酸 (SCFA) 改善肠道屏障功能，并且已经研究了个别 SCFA 的作用。在这里，研究了不同的 SCFA 混合物，代表纤维发酵产物的可能组成，以研究其对肠道屏障功能的保护和修复作用。四种膳食纤维（抗性淀粉、低聚果糖、高粱和玉米阿拉伯木聚糖）的发酵产物对屏障功能的影响与它们的 SCFA 浓度呈正相关。使用 Caco-2 细胞模型测试了不同浓度和组成的纯 SCFA 混合物。中等浓度（40-80mM）的 SCFA 可改善屏障功能，而不会对单层造成损害。在 40mM 的 SCFA 混合物中，丁酸比例为 20%和 50%对同时或在 SCFA 混合物之前施加的破坏剂（LPS/TNF-α）具有保护和修复单层的作用。将这一结果与膳食纤维选择相关联，缓慢发酵的纤维可以将适当浓度的 SCFA 递送到具有高比例丁酸的上皮细胞，从而改善屏障功能。
