Park S H, Hong C S
Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea.
Ann Allergy. 1989 Nov;63(5):405-10.
Allergen dot radioimmunoassay (dot-RIA), employing nitrocellulose filter paper (NFP) as the solid phase, and a Phadebas RAST tracer as the detecting antibody was used to measure the absolute amount of specific IgE to Dermatophagoides farinae (D. farinae) in forty-five D. farinae-sensitive patients and ten negative controls. These results with dot-RIA were compared with those with D. farinae radioallergosorbent test (RAST). When the elution of D. farinae-specific IgE from antibody-dotted NFP complex by alkaline buffer (pH 11.0) was done and followed by measurement of IgE contents in various dilutions of eluate to construct a standard curve, D. farinae-RAST exhibited greater binding than dot-RIA. There was a similar high degree of correlation of specific IgE levels between the ratio of specific IgE to total IgE by dot-RIA and RAST when compared with the wheal size on skin prick test to D. farinae. It was suggested that dot-RIA using NFP as the solid phase-a simple, less expensive, and reproducible method-is applicable for measuring the absolute level of specific IgE to D. farinae and for determining the relationship between the ratio of specific IgE to total IgE and the wheal size on skin prick test in D. farinae-sensitive patients.
采用硝酸纤维素滤纸（NFP）作为固相，以法玛西亚变应原吸附试验（Phadebas RAST）示踪剂作为检测抗体的变应原点状放射免疫测定法（dot-RIA），用于检测45例对粉尘螨敏感患者及10例阴性对照中针对粉尘螨（D. farinae）的特异性IgE的绝对量。将dot-RIA的这些结果与粉尘螨放射变应原吸附试验（RAST）的结果进行比较。当用碱性缓冲液（pH 11.0）从抗体包被的NFP复合物中洗脱粉尘螨特异性IgE，然后测量洗脱液不同稀释度中的IgE含量以构建标准曲线时，粉尘螨RAST显示出比dot-RIA更强的结合力。与粉尘螨皮肤点刺试验的风团大小相比，dot-RIA和RAST检测的特异性IgE与总IgE之比之间的特异性IgE水平具有相似的高度相关性。结果表明，使用NFP作为固相的dot-RIA是一种简单、成本较低且可重复的方法，适用于测量对粉尘螨敏感患者中针对粉尘螨的特异性IgE的绝对水平，以及确定特异性IgE与总IgE之比与皮肤点刺试验风团大小之间的关系。
