Qu Dovina, Subramony Siddarth D, Boskey Adele L, Pleshko Nancy, Doty Stephen B, Lu Helen H
Biomaterials and Interface Tissue Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York 10027.
Musculoskeletal Integrity Program, Hospital for Special Surgery, New York, New York 10021.
J Orthop Res. 2017 Nov;35(11):2513-2523. doi: 10.1002/jor.23539. Epub 2017 Mar 2.
The anterior cruciate ligament (ACL)-to-bone interface constitutes a complex, multi-tissue structure comprised of contiguous ligament, non-mineralized fibrocartilage, mineralized fibrocartilage, and bone regions. This composite structure enables load transfer between structurally and functionally dissimilar tissues and is critical for ligament homeostasis and joint stability. Presently, there is a lack of quantitative understanding of the matrix composition and organization across this junction, especially after the onset of skeletal maturity. The objective of this study is to characterize the adult bovine ACL-to-bone interface using Fourier transform infrared spectroscopic imaging (FTIRI), testing the hypothesis that regional changes in collagen, proteoglycan, and mineral distribution, as well as matrix organization, persist at the mature insertion. It was observed that while collagen content increases continuously across the adult interface, collagen alignment decreases between ligament and bone. Proteoglycans were primarily localized to the fibrocartilage region and an exponential increase in mineral content was observed between the non-mineralized and mineralized regions. These observations reveal significant changes in collagen distribution and alignment with maturity, and these trends underscore the role of physiologic loading in postnatal matrix remodeling. Findings from this study provide new insights into interface organization and serve as benchmark design criteria for interface regeneration and integrative soft tissue repair. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:2513-2523, 2017.
前交叉韧带（ACL）与骨的界面构成了一个复杂的多组织结构，由相邻的韧带、非矿化纤维软骨、矿化纤维软骨和骨区域组成。这种复合结构能够在结构和功能不同的组织之间进行负荷传递，对于韧带的稳态和关节稳定性至关重要。目前，对于这个连接处的基质组成和组织结构缺乏定量的了解，尤其是在骨骼成熟之后。本研究的目的是使用傅里叶变换红外光谱成像（FTIRI）对成年牛ACL与骨的界面进行表征，检验这样一个假设：在成熟的附着点处，胶原蛋白、蛋白聚糖和矿物质分布以及基质组织的区域变化仍然存在。研究发现，虽然成年界面上的胶原蛋白含量持续增加，但韧带和骨之间的胶原蛋白排列减少。蛋白聚糖主要定位于纤维软骨区域，并且在非矿化区域和矿化区域之间观察到矿物质含量呈指数增加。这些观察结果揭示了胶原蛋白分布和排列随成熟度的显著变化，这些趋势强调了生理负荷在出生后基质重塑中的作用。本研究的结果为界面组织提供了新的见解，并作为界面再生和整合性软组织修复的基准设计标准。© 2017骨科学研究协会。由威利期刊公司出版。《矫形外科学研究杂志》35:2513 - 2523，2017年。
