Bhandari Jaya, Muhammad BushraTaj, Thapa Pratiksha, Shrestha Bhupal Govinda
Department of Biotechnology, Kathmandu University, Kathmandu, Nepal.
Panjwani Centre for Molecular Medicine and Drug Research, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
BMC Complement Altern Med. 2017 Feb 8;17(1):102. doi: 10.1186/s12906-017-1622-6.
There is growing interest in the use of plants for the treatment and prevention of cancer. Medicinal plants are currently being evaluated as source of promising anticancer agents. In this paper, we have investigated the anticancer potential of plant Allium wallichii, a plant native to Nepal and growing at elevations of 2300-4800 m. This is the first study of its kind for the plant mentioned.
The dried plant was extracted in aqueous ethanol. Phytochemical screening, anti-microbial assay, anti-oxidant assay, cytotoxicity assay and the flow-cytometric analysis were done for analyzing different phytochemicals present, anti-microbial activity, anti-oxidant activity and anti-cancer properties of Allium wallichii.
We observed the presence of steroids, terpenoids, flavonoids, reducing sugars and glycosides in the plant extract and the plant showed moderate anti-microbial and anti-oxidant activity. The IC values of Allium wallichii in different cancer cell lines are 69.69 μg/ml for Prostate cancer (PC3) cell line, 55.29 μg/ml for Breast Cancer (MCF-7) cell line and 46.51 μg/ml for cervical cancer (HeLa) cell line as compared to Doxorubicin (0.85 μg/ml). The cell viability assay using FACS showed that the ICvalue of Allium wallichii for Burkitt's lymphoma (B-Lymphoma) cell line was 3.817 ± 1.99 mg/ml.
Allium wallichii can be an important candidate to be used as an anticancer agent. Separation of pure compounds with bioassay guided extraction, spectrometric analysis and subsequent cytotoxicity assay of the pure bioactive compounds from Allium wallichii is highly recommended as the crude extract itself showed promising cytotoxicity.
植物在癌症治疗和预防中的应用正受到越来越多的关注。药用植物目前正作为有前景的抗癌药物来源进行评估。在本文中，我们研究了尼泊尔本土、生长于海拔2300 - 4800米的植物棱叶韭的抗癌潜力。这是针对该植物的首例此类研究。
将干燥的植物用乙醇水溶液提取。进行了植物化学筛选、抗菌测定、抗氧化测定、细胞毒性测定和流式细胞术分析，以分析棱叶韭中存在的不同植物化学物质、抗菌活性、抗氧化活性和抗癌特性。
我们在植物提取物中观察到甾体、萜类、黄酮类、还原糖和糖苷的存在，并且该植物表现出适度的抗菌和抗氧化活性。与阿霉素（0.85μg/ml）相比，棱叶韭在不同癌细胞系中的IC值分别为：前列腺癌（PC3）细胞系69.69μg/ml，乳腺癌（MCF - 7）细胞系55.29μg/ml，宫颈癌（HeLa）细胞系46.51μg/ml。使用流式细胞仪进行的细胞活力测定表明，棱叶韭对伯基特淋巴瘤（B -淋巴瘤）细胞系的IC值为3.817±1.99mg/ml。
棱叶韭可以成为一种重要的抗癌药物候选物。强烈建议通过生物测定引导的提取、光谱分析以及随后对棱叶韭纯生物活性化合物进行细胞毒性测定来分离纯化合物，因为粗提物本身就显示出有前景的细胞毒性。
