Department of Biotechnology, University of North Bengal, Raja Rammohunpur, Darjeeling, West Bengal, 734013, India.
BMC Complement Altern Med. 2019 Aug 5;19(1):200. doi: 10.1186/s12906-019-2621-6.
Astilbe rivularis L. is an indigenous medicinal plant growing in high altitude of Darjeeling Himalayan region of India and Nepal. The plant rhizome has been used traditionally as medicine by local tribes to treat various ailments including infectious and other diseases. The present study aims to evaluate the plant rhizome for chemical composition and in vitro antioxidant, antibacterial and cytotoxic bioactivities.
The methanolic extract of rhizome was analyzed for phytochemical constituents by biochemical and GC-MS methods. The antibacterial property of the extract was monitored by agar well diffusion assay. Antioxidant potential was assessed by in vitro DPPH and ABTS scavenging assays and scavenging of induced ROS in normal cell line using fluorescent probe 2', 7'- dichlorofluorescin diacetate. Cytotoxic effect of the extract in cancer and normal cell lines was determined by MTT assay.
Rhizome methanolic extract contained terpenoids, flavonoids, tannins, phenols, alkaloids, saponins and reducing sugars. Further analysis of extract by GC-MS showed the presence of nine major constituents belonging to terpenoids and fatty acid groups. The extract had marked in vitro ROS scavenging activity and moderate antibacterial activity against gram positive and gram negative bacteria. It showed cytotoxicity to neuroblastoma (SHSY5Y) cell line with IC value < 100 μg ml but had least damaging effect on normal cells, like human embryonic kidney (HEK-293) and liver (WRL-68) cell lines.
The study suggests that Astilbe rivularis has potential as source of new potent antibacterial, antioxidant and anticancer agents. Further studies on purification and characterization of active compounds from Astilbe rivularis and their biological evaluation are highly recommended.
蛇头七（Astilbe rivularis L.）是一种生长在印度大吉岭喜马拉雅地区和尼泊尔高海拔地区的本土药用植物。该植物的根茎在传统上被当地部落用作药物，用于治疗各种疾病，包括传染病和其他疾病。本研究旨在评估该植物根茎的化学成分以及体外抗氧化、抗菌和细胞毒性生物活性。
采用生化和 GC-MS 方法分析根茎的甲醇提取物中的植物化学成分。通过琼脂孔扩散法监测提取物的抗菌性能。通过体外 DPPH 和 ABTS 清除试验以及荧光探针 2',7'-二氯荧光素二乙酸酯检测诱导的 ROS 在正常细胞系中的清除来评估抗氧化潜力。通过 MTT 试验测定提取物在癌细胞和正常细胞系中的细胞毒性作用。
根茎甲醇提取物含有萜类、黄酮类、单宁、酚类、生物碱、皂苷和还原糖。通过 GC-MS 对提取物进一步分析表明，存在属于萜类和脂肪酸组的 9 种主要成分。该提取物具有显著的体外 ROS 清除活性和对革兰氏阳性和革兰氏阴性菌的适度抗菌活性。它对神经母细胞瘤（SHSY5Y）细胞系表现出细胞毒性，IC 值<100μg/ml，但对正常细胞（如人胚肾（HEK-293）和肝（WRL-68）细胞系）的损伤最小。
研究表明，蛇头七具有作为新的有效抗菌、抗氧化和抗癌药物来源的潜力。建议进一步研究从蛇头七中纯化和鉴定活性化合物及其生物学评价。
