Deng Yu, Zhang Xi, Zhang Xiaojuan
National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China.
Appl Microbiol Biotechnol. 2017 Mar;101(6):2217-2226. doi: 10.1007/s00253-017-8156-1. Epub 2017 Feb 9.
Actinobacteria are extremely important to human health, agriculture, and forests. Because of the vast differences of the characteristics of Actinobacteria, a lot of genetic tools have been developed for efficiently manipulating the genetics. Although there are a lot of successful examples of engineering Actinobacteria, they are still more difficult to be genetically manipulated than other model microorganisms such as Saccharomyces cerevisiae, Escherichia coli, and Bacillus subtilis etc. due to the diverse genomics and biochemical machinery. Here, we review the methods to introduce heterologous DNA into Actinobacteria and the available genetic modification tools. The trends and problems existing in engineering Actinobacteria are also covered.
放线菌对人类健康、农业和森林极为重要。由于放线菌特性差异巨大，已开发出许多遗传工具用于高效操纵其遗传学特性。尽管有许多改造放线菌的成功实例，但由于其基因组学和生化机制多样，与酿酒酵母、大肠杆菌和枯草芽孢杆菌等其他模式微生物相比，放线菌的遗传操作仍更为困难。在此，我们综述了将异源DNA导入放线菌的方法以及可用的遗传修饰工具。还涵盖了放线菌工程中存在的趋势和问题。
