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[利用分子生物学方法进行痕迹中的物种鉴定]

[Species identification in traces using molecular biology methods].

作者信息

Teifel-Greding J, Baur C, Josephi E, Liebhardt E

出版信息

Beitr Gerichtl Med. 1989;47:545-50.

PMID:2818530
Abstract

The species of a biological sample (blood, hairs) was determined by hybridization of its DNA with specific control DNA using either the former or the latter as probe. The labelling of the probes occurred with radioactive nucleotides by random priming or by the non-radioactive sulfonation of cytosine residues. Species identification by means of DNA hybridization is practicable even after denaturing the sample by heat or after treatment with H2O2. In case of a human sample, the sex of the donor can be determined, too.

摘要

通过将生物样本(血液、毛发)的DNA与特定对照DNA杂交,并以前者或后者作为探针来确定生物样本的物种。探针通过随机引物用放射性核苷酸标记,或通过胞嘧啶残基的非放射性磺化进行标记。即使在通过加热使样本变性或用过氧化氢处理后,通过DNA杂交进行物种鉴定也是可行的。对于人类样本,也可以确定供体的性别。

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