Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornova, Izmir, Turkey; Department of Genetic & Bioengineering, Faculty of Engineering, Gumushane University, 29100 Gumushane, Turkey.
Hamburg University of Technology, Institute of Thermal Separation Processes, Eißendorferstr. 38, D 21073 Hamburg, Germany.
Carbohydr Polym. 2017 Apr 1;161:228-234. doi: 10.1016/j.carbpol.2017.01.004. Epub 2017 Jan 9.
The objective of this study was to develop an injectable alginate based formulation for immobilizing enzymes into microfluidic systems. The gelation was induced upon lowering the pH by addition of d-glucono-δ-lactone (GDL) and release of Ca ions from solid CaCO. The effects of GDL concentration on enzymatic activity and gelation time were investigated. The results indicated that increasing the GDL concentration increased both surface area and enzymatic activity. Also, chitosan was added to the formulation at different ratios to enhance the stability of enzyme during immobilization. For microfluidic application, 100μl spiral coil single channel microchip was fabricated and alginate GDL mixture containing β-glucosidase was injected to the microchannel prior to gelation. Enzymatic conversion was performed by pumping substrate (pNPG) through the microchannel. The results indicated that the entire substrate was converted continuously during 24h without any leakage or deactivation of immobilized enzyme.
本研究旨在开发一种可注射的海藻酸钠制剂，用于将酶固定在微流控系统中。通过添加 d-葡萄糖酸-δ-内酯（GDL）降低 pH 值并从固体 CaCO 中释放 Ca 离子来引发凝胶化。考察了 GDL 浓度对酶活性和凝胶时间的影响。结果表明，增加 GDL 浓度可以增加酶的比表面积和活性。此外，还向制剂中添加了不同比例的壳聚糖，以增强酶在固定化过程中的稳定性。对于微流控应用，制造了 100μl 螺旋盘管单通道微芯片，并在凝胶化之前将含有β-葡萄糖苷酶的海藻酸钠 GDL 混合物注入微通道。通过微通道泵送底物（pNPG）进行酶转化。结果表明，在 24 小时内，整个底物连续转化，没有固定化酶的任何泄漏或失活。
