Jiang Jie-Lan, Mao Ming-Guang, Lü Hui-Qian, Wen Shi-Hui, Sun Meng-Lei, Liu Rui-Ting, Jiang Zhi-Qiang
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2017 Dec;24:12-18. doi: 10.1016/j.cbd.2017.05.003. Epub 2017 May 30.
The adverse effects of hypoxia are confined to biochemical, physiological, developmental and behavioral processes, especially injury of the brain. In this study, a subset of genes in the brain of Takifugu rubripes were analyzed using digital gene expression (DGE) profiles and next-generation sequencing after acute hypoxia. Among 32 differentially expressed genes, 29 were up-regulated and 3 were down-regulated following hypoxia exposure. Using Gene Ontology analysis, it was found that transcription and translation, metabolism, and the stress response were affected by exposure to hypoxia. KEGG analysis revealed that the neuroactive ligand-receptor interaction pathway was significantly enriched in hypoxia-exposed T. rubripes. To further confirm the differential expression of genes, quantitative real-time PCR was performed to test six candidate genes, with the following five genes exhibiting the same expression patterns as the sequencing results: Proto-oncogene c-fos, Kruppel-like factor 2, immediate early response 2, proopiomelanocortin A and rhodopsin. This work is the first to identify and annotate genes in T. rubripes affected by hypoxia stress. This investigation provides data for understanding the molecular mechanism of fish adaptation to hypoxia and provides a reference for rationally setting dissolved oxygen levels in aquaculture.
缺氧的不良影响仅限于生物化学、生理、发育和行为过程，尤其是对大脑的损伤。在本研究中，利用数字基因表达（DGE）谱和下一代测序技术，对急性缺氧后红鳍东方鲀大脑中的一部分基因进行了分析。在32个差异表达基因中，缺氧暴露后有29个基因上调，3个基因下调。通过基因本体分析发现，转录与翻译、代谢以及应激反应均受到缺氧暴露的影响。京都基因与基因组百科全书（KEGG）分析显示，在缺氧暴露的红鳍东方鲀中，神经活性配体-受体相互作用途径显著富集。为进一步确认基因的差异表达，进行了定量实时PCR以检测6个候选基因，其中以下5个基因表现出与测序结果相同的表达模式：原癌基因c-fos、类 Kruppel 样因子2、即刻早期反应基因2、阿黑皮素原A和视紫红质。这项工作首次鉴定并注释了受缺氧应激影响的红鳍东方鲀基因。本研究为理解鱼类适应缺氧的分子机制提供了数据，并为合理设定水产养殖中的溶解氧水平提供了参考。
