Xiong Lei, Dong Jinxiu, Jiang Hui, Zan Jiawei, Tong Jiucui, Liu Jianjun, Wang Meng, Nie Liuwang
Life Science College of Anhui Normal University, Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, P.R. China.
Biochemistry Department of Wannan Medical College, Provincial Key Laboratory of Biological Macro-molecules Research, Wuhu, Anhui, P.R. China.
PeerJ. 2019 Mar 8;7:e6557. doi: 10.7717/peerj.6557. eCollection 2019.
is a classical organism with temperature-dependent sex determination (TSD). Gonad development in early life has recently received considerable attention but gonadal maintenance after sex differentiation in turtles with TSD remains a mystery. In this study, we sequenced the transcriptomes for the adult testis and ovary using RNA-seq, and 36,221 transcripts were identified. In total, 1,594 differentially expressed genes (DEGs) were identified where 756 DEGs were upregulated in the testis and 838 DEGs were upregulated in the ovary. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis suggested that the TGF-beta signaling pathway and Hedgehog signaling pathway have important roles in testis maintenance and spermatogenesis, whereas the Hippo signaling pathway and Wnt signaling pathway are likely to participate in ovary maintenance. We determined the existence of antagonistic networks containing significant specific-expressed genes and pathways related to gonadal maintenance and gametogenesis in the adult gonads of . The candidate gene Fibronectin type 3 and ankyrin repeat domains 1 () might be involved with the regulation of testis spermatogenesis.
是一种具有温度依赖型性别决定（TSD）的经典生物。早期生命阶段的性腺发育最近受到了相当多的关注，但TSD海龟性别分化后的性腺维持仍然是一个谜。在本研究中，我们使用RNA测序对成年睾丸和卵巢的转录组进行了测序，共鉴定出36,221个转录本。总共鉴定出1594个差异表达基因（DEG），其中756个DEG在睾丸中上调，838个DEG在卵巢中上调。基因本体论和京都基因与基因组百科全书通路分析表明，TGF-β信号通路和刺猬信号通路在睾丸维持和精子发生中起重要作用，而河马信号通路和Wnt信号通路可能参与卵巢维持。我们确定了在成年性腺中存在包含与性腺维持和配子发生相关的显著特异性表达基因和通路的拮抗网络。候选基因纤连蛋白3型和锚蛋白重复结构域1（）可能参与睾丸精子发生的调控。
