Benatti Lucia, Corvi Federico, Tomasso Livia, Mercuri Stefano, Querques Lea, Ricceri Fulvio, Bandello Francesco, Querques Giuseppe
Department of Ophthalmology, University Vita-Salute, IRCCS Ospedale San Raffaele, Via Olgettina 60, Milan, 20132, Italy.
Graefes Arch Clin Exp Ophthalmol. 2017 Jun;255(6):1079-1083. doi: 10.1007/s00417-017-3602-4. Epub 2017 Feb 11.
To analyze the inter-methods agreement in arteriovenous ratio (AVR) evaluation between spectral-domain optical coherence tomography (SD-OCT) and Dynamic Vessel Analyzer (DVA).
Healthy volunteers underwent DVA and SD-OCT examination. AVR was measured by SD-OCT using the four external lines of the optic nerve head-centered 7-line cube and by DVA using an automated AVR estimation. The mean AVR was calculated, twice, separately by two independent readers for each tool.
Twenty-two eyes of 11 healthy subjects (five women and six men, mean age 35) were included. AVR analysis by DVA showed high inter-observer agreement between reader 1 and 2, and high intra-observer agreement for both reader 1 and reader 2. With regard to AVR analysis on SD-OCT, we found high inter-observer agreement between reader 1 and 2, and low intra-observer agreement for reader 2 but high intra-observer agreement for reader 1. Overall, the mean AVR measured on SD-OCT turned out to be significantly higher than mean AVR measured through DVA (reader 1, 0.9023 ± 0.06 vs 0.8036 ± 0.08; p < 0.001, and reader 2, 0.9067 ± 0.06 vs 0.8083 ± 0.05; p= 0.003).
No inter-method agreement in AVR could be detected in the present study due to bias in measurements (shift between DVA and SD-OCT). We found significant difference in the two noninvasive methods for AVR measurement, with a tendency for SD-OCT to overestimate retinal vascular caliber in comparison to DVA. This may be useful for achieving greater accuracy in the evaluation of retinal vessel in ocular as well as systemic diseases.
分析光谱域光学相干断层扫描（SD - OCT）和动态血管分析仪（DVA）在动静脉比率（AVR）评估中的方法间一致性。
健康志愿者接受DVA和SD - OCT检查。通过SD - OCT使用以视神经乳头为中心的7线立方体的四条外线测量AVR，通过DVA使用自动AVR估计值测量AVR。由两名独立读者分别对每种工具计算两次平均AVR。
纳入11名健康受试者（5名女性和6名男性，平均年龄35岁）的22只眼。DVA进行的AVR分析显示读者1和读者2之间观察者间一致性高，读者1和读者2的观察者内一致性也高。关于SD - OCT上的AVR分析，我们发现读者1和读者2之间观察者间一致性高，读者2的观察者内一致性低，但读者1的观察者内一致性高。总体而言，SD - OCT测量的平均AVR显著高于通过DVA测量的平均AVR（读者1，0.9023±0.06对0.8036±0.08；p < 0.001，读者2，0.9067±0.06对0.8083±0.05；p = 0.003）。结论：由于测量偏差（DVA和SD - OCT之间的偏移），本研究未检测到AVR的方法间一致性。我们发现两种非侵入性AVR测量方法存在显著差异，与DVA相比，SD - OCT有高估视网膜血管管径的趋势。这可能有助于在眼部及全身性疾病的视网膜血管评估中实现更高的准确性。
