Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, China.
Key Laboratory of Carbohydrate Science and Engineering, Chongqing Normal University, Chongqing 401331, China.
Ultrason Sonochem. 2024 Oct;109:107010. doi: 10.1016/j.ultsonch.2024.107010. Epub 2024 Jul 31.
Purple mangosteen scarfskin polysaccharide has many important physiological functions, but its preparation method, structure, and function need further exploration. A polysaccharide was obtained from mangosteen scarfskin by ultrasonic-assisted extraction and purified. On this basis, its structure and physicochemical properties were investigated. The Congo red experiment was used to determine whether it has a triple helix conformation. The structure of purple mangosteen scarfskin polysaccharide was further analyzed by infrared spectroscopy and nuclear magnetic analysis. The antioxidant activities of the above three polysaccharides were studied by related experiments. It was found that the monosaccharide composition of purple mangosteen scarfskin polysaccharide mainly contained a large amount of arabinose, a small amount of rhamnoose and a very small amount of galacturonic acid, and its core main chain was composed of 1,4-α-arabinose. It did not have this spatial configuration. After the acetylation of purple mangosteen scarfskin polysaccharide, the acetylated derivative with a degree of substitution of 0.33 was obtained. It was found that they had certain scavenging and inhibiting effects on hydroxyl radicals and lipid peroxidation, and their activities were related to the concentration of polysaccharides. Meanwhile, the antioxidant activity of the polysaccharide was significantly enhanced after the modified treatment of acetylation, which indicated that chemical modification could effectively improve some activities of polysaccharide. The above studies provided some reference value for the further research and development of purple mangosteen scarfskin polysaccharide.
山竹果皮多糖具有许多重要的生理功能，但它的制备方法、结构和功能仍需要进一步探索。本研究采用超声辅助提取法从山竹果皮中提取并纯化得到一种多糖，在此基础上对其结构和理化性质进行了研究。刚果红实验用于确定其是否具有三螺旋构象。通过红外光谱和核磁共振分析进一步分析了山竹果皮多糖的结构。通过相关实验研究了上述三种多糖的抗氧化活性。结果表明，山竹果皮多糖的单糖组成主要含有大量的阿拉伯糖、少量的鼠李糖和极少量的半乳糖醛酸，其核心主链由 1,4-α-阿拉伯糖组成。它没有这种空间构型。山竹果皮多糖经过乙酰化修饰后，得到取代度为 0.33 的乙酰化衍生物。结果表明，它们对羟基自由基和脂质过氧化具有一定的清除和抑制作用，其活性与多糖浓度有关。同时，乙酰化修饰后的多糖抗氧化活性显著增强，这表明化学修饰可以有效提高多糖的某些活性。上述研究为进一步研究和开发山竹果皮多糖提供了一定的参考价值。
