Department of Chemistry, College of Natural Sciences, Hanyang University , Seoul, 04763, Republic of Korea.
Anal Chem. 2017 Feb 21;89(4):2449-2456. doi: 10.1021/acs.analchem.6b04418. Epub 2017 Feb 6.
There has been a great deal of research regarding the cellular association of nanoparticles (NPs), although there are only a few methods available yet for the quantitative measurements of cellular NPs. In this study, we propose a simple and quantitative method to estimate the cellular uptake of Au NPs into cervical cancer cells (HeLa) based on their side scattering (SSC) intensities measured by flow cytometry (FCM). We have compared SSC intensities of HeLa cells exposed to eight different types of Au NPs (40-100 nm size, with positive or negative surface charge) with the amount of cellular Au NPs measured by inductively coupled plasma mass spectrometry (ICPMS). On the basis of these comparisons, we have found linear correlations between the cellular Au NPs and the SSC intensities and used them to estimate the amount of Au NPs associated with HeLa cells. Once the correlations were found for specific cell lines and types of nanoparticles, this approach is useful for simple and quantitative estimation of the cellular Au NPs, without performing labor-intensive and complicated sample preparation procedures required for the ICPMS approach.
尽管已经有很多关于纳米粒子（NPs）细胞相关性的研究，但目前仅有少数几种方法可用于定量测量细胞内的 NPs。在这项研究中，我们提出了一种简单而定量的方法，基于流式细胞术（FCM）测量的侧向散射（SSC）强度，来估计宫颈癌细胞（HeLa）摄取 Au NPs 的情况。我们比较了暴露于 8 种不同类型的 Au NPs（40-100nm 大小，带正或负表面电荷）的 HeLa 细胞的 SSC 强度与电感耦合等离子体质谱法（ICPMS）测量的细胞内 Au NPs 量。基于这些比较，我们发现了细胞内 Au NPs 与 SSC 强度之间的线性相关性，并利用它们来估计与 HeLa 细胞相关的 Au NPs 量。一旦针对特定细胞系和类型的纳米粒子找到了相关性，这种方法就可以用于简单而定量地估计细胞内的 Au NPs，而无需进行 ICPMS 方法所需的繁琐而复杂的样品制备程序。
