Radiobiology Laboratory, Department of Medical Physics, Greater Poland Cancer Centre, 61-866 Poznań, Poland.
Department of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, 61-614 Poznań, Poland.
Int J Mol Sci. 2020 Dec 22;22(1):16. doi: 10.3390/ijms22010016.
To increase the efficiency of therapy via enhancing its selectivity, the usage of gold nanorods (GNR) as a factor sensitizing cancer cells to radiation was proposed. Due to gold nanoparticles' characteristics, the smaller doses of radiation would be sufficient in the treatment, protecting the healthy tissue around the tumor. The aim of this study was to investigate the effect of gold nanorods on cancer and normal prostate cells and the role of nanorods in the cell response to ionizing radiation. The effect was evaluated by measuring the toxicity, cell cycle, cell granularity, reactive oxygen species (ROS) level, and survival fractions. Nanorods showed a strong toxicity dependent on the concentration and incubation time toward all used cell lines. A slight effect of nanorods on the cycle distribution was observed. The results demonstrated that the administration of nanorods at higher concentrations resulted in an increased level of generated radicals. The results of cellular proliferation after irradiation are ambiguous; however, there are noticeable differences after the application of nanorods before irradiation. The obtained results lead to the conclusion that nanorods affect the physiology of both normal and cancer cells. Nanorods might become a potential tool used to increase the effectiveness of radiation treatment.
为了提高治疗的效率，增强其选择性，提出了将金纳米棒（GNR）用作使癌细胞对辐射敏感的因素。由于金纳米粒子的特性，较小剂量的辐射就足以进行治疗，保护肿瘤周围的健康组织。本研究的目的是研究金纳米棒对癌细胞和正常前列腺细胞的影响，以及纳米棒在细胞对电离辐射的反应中的作用。通过测量毒性、细胞周期、细胞粒度、活性氧（ROS）水平和存活分数来评估效果。纳米棒对所有使用的细胞系都表现出强烈的、浓度和孵育时间依赖性的毒性。观察到纳米棒对细胞周期分布有轻微影响。结果表明，在较高浓度下给予纳米棒会导致产生的自由基水平增加。照射后细胞增殖的结果是模棱两可的；然而，在照射前应用纳米棒后会有明显的差异。获得的结果得出结论，纳米棒会影响正常和癌细胞的生理机能。纳米棒可能成为提高辐射治疗效果的潜在工具。
