Lee Min Young, Kim Hyun A, Kang Myung Hee
Department of Food Science and Nutrition, Daedeok Valley Campus, Hannam University, 461-6 Jeonmin-dong, Yuseng-gu, Daejeon 34054, Korea.
Nutr Res Pract. 2017 Feb;11(1):33-42. doi: 10.4162/nrp.2017.11.1.33. Epub 2017 Jan 20.
BACKGROUND/OBJECTIVE: This study aims to measure the antioxidant capacity of Korean diet (KD) with American diet (AD) as a control group and to examine the DNA damage reduction effect on human lymphocytes.
MATERIALS/METHODS: The KD applied in this study is the standard one-week meals for Koreans (2,000 kcal/day) suggested by 2010 Dietary Reference Intakes for Koreans. The AD, which is the control group, is a one-week menu (2,000 kcal/day) that consists of foods that Americans would commonly take in according to the National Health and Nutrition Examination Survey. The antioxidant capacity of each menu was measured by means of the total phenolic assay and 3 antioxidant activity assays (2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, trolox equivalent antioxidant capacity (TEAC), Oxygen radical absorbance capacity (ORAC)), while the extent of lymphocyte DNA damage was measured by means of the comet assay.
When measured by means of TEAC assay, the antioxidant capacity of the KD of the day was higher than that of the AD ( < 0.05) while there was no significant difference in total phenolic contents and DPPH and ORAC assays. The lymphocyte DNA damage protective effect of the KD was significantly higher than that of the AD ( < 0.01). As for the one-week menu combining the menus for 7 days, the total phenolic assay ( < 0.05) and antioxidant capacity ( < 0.001, DPPH; < 0.01, TEAC) of the KD menu were significantly higher than those of the AD menu. Likewise, the DNA damage reduction rate of the Korean seven-day menu was significantly higher than that of the American menu ( < 0.01).
This study demonstrates that the high antioxidant capacity and DNA damage protective effect of KD, which consists generally of various plant foods, are higher than those of typical AD.
背景/目的：本研究旨在以美国饮食（AD）作为对照组，测定韩国饮食（KD）的抗氧化能力，并研究其对人类淋巴细胞DNA损伤的减轻作用。
材料/方法：本研究中使用的KD是2010年韩国膳食参考摄入量建议的韩国人标准一周膳食（2000千卡/天）。作为对照组的AD是根据国家健康与营养检查调查，由美国人通常食用的食物组成的一周菜单（2000千卡/天）。通过总酚含量测定和3种抗氧化活性测定（2,2-二苯基-1-苦基肼（DPPH）自由基清除活性、特洛克斯当量抗氧化能力（TEAC）、氧自由基吸收能力（ORAC））来测量每个菜单的抗氧化能力，同时通过彗星试验测量淋巴细胞DNA损伤程度。
通过TEAC测定法测量时，当日KD的抗氧化能力高于AD（P<0.05），而总酚含量以及DPPH和ORAC测定中无显著差异。KD对淋巴细胞DNA损伤的保护作用显著高于AD（P<0.01）。对于将7天菜单组合而成的一周菜单，KD菜单的总酚含量测定（P<0.05）和抗氧化能力（DPPH，P<0.001；TEAC，P<0.01）显著高于AD菜单。同样，韩国七日菜单的DNA损伤减少率显著高于美国菜单（P<0.01）。
本研究表明，通常由各种植物性食物组成的KD具有较高的抗氧化能力和DNA损伤保护作用，高于典型的AD。
