Yan Bo-Jing, Wu Zhi-Zhong, Chong Wei-Hua, Li Gen-Lin
Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology & Visual Sciences Key Lab, Beijing, China.
Neural Regen Res. 2016 Dec;11(12):1981-1989. doi: 10.4103/1673-5374.197142.
Several studies have investigated the protective functions of brain-derived neurotrophic factor (BDNF) in retinitis pigmentosa. However, a BDNF-based therapy for retinitis pigmentosa is not yet available. To develop an efficient treatment for fundus disease, an eukaryotic expression plasmid was generated and used to transfect human 293T cells to assess the expression and bioactivity of BDNF on acute retinal pigment epithelial-19 (ARPE-19) cells, a human retinal epithelial cell line. After 96 hours of co-culture in a Transwell chamber, ARPE-19 cells exposed to BDNF secreted by 293T cells were more viable than ARPE-19 cells not exposed to secreted . Western blot assay showed that Bax levels were downregulated and that Bcl-2 levels were upregulated in human ARPE-19 cells exposed to BDNF. Furthermore, 293T cells transfected with the BDNF gene steadily secreted the protein. The powerful anti-apoptotic function of this BDNF may be useful for the treatment of retinitis pigmentosa and other retinal degenerative diseases.
多项研究已对脑源性神经营养因子（BDNF）在色素性视网膜炎中的保护作用进行了调查。然而，尚未有基于BDNF的色素性视网膜炎治疗方法。为开发一种针对眼底疾病的有效治疗方法，构建了一种真核表达质粒，并用于转染人293T细胞，以评估BDNF在人视网膜上皮细胞系急性视网膜色素上皮-19（ARPE-19）细胞上的表达及生物活性。在Transwell小室中共培养96小时后，暴露于293T细胞分泌的BDNF的ARPE-19细胞比未暴露于分泌的BDNF的ARPE-19细胞更具活力。蛋白质印迹分析表明，暴露于BDNF的人ARPE-19细胞中Bax水平下调，Bcl-2水平上调。此外，用BDNF基因转染的293T细胞稳定分泌该蛋白。这种BDNF强大的抗凋亡功能可能对色素性视网膜炎和其他视网膜退行性疾病的治疗有用。
