Xiao Xiaoping, Liu Yaoran, Guo Zhengguang, Liu Xiaoyan, Sun Haidan, Li Qian, Sun Wei
Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China.
Department of stomatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
Proteomics Clin Appl. 2017 Jul;11(7-8). doi: 10.1002/prca.201600142. Epub 2017 Mar 21.
Human saliva is an important source for disease biomarker discovery. This study is to investigate the influence of gender and acid stimulation on the normal human salivary proteome.
Unstimulated and acid-stimulated saliva samples from 5 males and 5 females were labeled with 4-plex iTRAQ and analyzed by 2-DLC MS/MS. By bioinformatics analysis the gender and acid stimulation related proteins were defined. According to protein annotation the important proteins were validated by multiple reaction monitor analysis.
A total of 1770 proteins were identified, and 82 proteins in unstimulated saliva were found to be gender-specific, mainly associated with immune function, metabolism and inflammation. However, no gender-specific proteins were found in acid-stimulated saliva. In addition, 182 and 307 differential proteins were found to be acid stimulation-specific in male samples and female samples, respectively, mainly participated in the process of cellular movement, immune function and inflammatory response. Besides, it was found that acid stimulation caused more significant alteration and played a more important role in the human salivary proteome than gender. Gender-specific (IGHG2 and TIMP1) and acid stimulation (PERL, ENOA, ACTB, B4E022 and CALL3) related proteins were validated by MRM analysis.
The results indicate that gender differences exist in the unstimulated salivary proteome, and the influence of acid stimulation on the salivary proteome was more significant than that of gender. The above results may be helpful for salivary proteome research in the future.
人类唾液是疾病生物标志物发现的重要来源。本研究旨在探讨性别和酸刺激对正常人唾液蛋白质组的影响。
对5名男性和5名女性的未刺激唾液和酸刺激唾液样本进行4重iTRAQ标记，并通过二维液相色谱串联质谱进行分析。通过生物信息学分析确定与性别和酸刺激相关的蛋白质。根据蛋白质注释，通过多反应监测分析对重要蛋白质进行验证。
共鉴定出1770种蛋白质，发现未刺激唾液中有82种蛋白质具有性别特异性，主要与免疫功能、代谢和炎症相关。然而，在酸刺激唾液中未发现性别特异性蛋白质。此外，分别在男性样本和女性样本中发现182种和307种酸刺激特异性差异蛋白质，主要参与细胞运动、免疫功能和炎症反应过程。此外，发现酸刺激在人类唾液蛋白质组中引起的变化比性别更显著，且作用更重要。通过多反应监测分析验证了与性别特异性（IGHG2和TIMP1）和酸刺激（PERL、ENOA、ACTB、B4E022和CALL3）相关的蛋白质。
结果表明，未刺激唾液蛋白质组存在性别差异，酸刺激对唾液蛋白质组的影响比性别更显著。上述结果可能有助于未来的唾液蛋白质组研究。
