Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, 7000-083 Évora, Portugal.
Departamento de Medicina Veterinária, Escola de Ciências e Tecnologia, Universidade de Évora, 7000-083 Évora, Portugal.
Biomed Res Int. 2018 Jun 3;2018:7456894. doi: 10.1155/2018/7456894. eCollection 2018.
Saliva gained interest as a potential noninvasive source of biomarkers in humans and that interest starts to be extended also to other animal species. For this purpose, the knowledge of the salivary proteome in healthy conditions and the factors that affect it and how they affect it are necessary. The aim of the present study was to assess the effect that gender and breed have in saliva proteome and the changes in it induced by stimulation with acid. Saliva from 4 different purebred dogs (Portuguese Podengo, Greyhound, Rafeiro Alentejano, and Beagle) of both genders was collected without and after stimulation with lemon juice. SDS-PAGE and two-dimensional gel electrophoresis (2-DE) profiles were compared and the proteins of interest in-gel digested and identified by mass spectrometry. Acid stimulation decreased total protein concentration and the relative amounts of some protein bands/spots. Gender appeared to have minimal effect in saliva proteome, whereas the influence of breed varies. Beagles and Portuguese Podengos were the two breeds with higher differences. In conclusion, stimulation procedures and dog breed should be considered in data analysis when using salivary proteins for diagnostic purposes.
唾液作为一种有潜力的非侵入性人类生物标志物来源引起了人们的兴趣，这种兴趣也开始扩展到其他动物物种。为此，有必要了解健康状况下的唾液蛋白质组以及影响它的因素及其影响方式。本研究的目的是评估性别和品种对唾液蛋白质组的影响，以及酸刺激引起的变化。收集了来自 4 种不同纯种狗（葡萄牙波登戈犬、格雷伊猎犬、拉夫里奥·阿连特茹犬和比格犬）的雌雄唾液，在没有和用柠檬汁刺激后进行收集。比较 SDS-PAGE 和二维凝胶电泳（2-DE）图谱，并对凝胶中感兴趣的蛋白质进行消化和质谱鉴定。酸刺激降低了总蛋白浓度和一些蛋白质带/斑点的相对含量。性别对唾液蛋白质组的影响很小，而品种的影响则不同。比格犬和葡萄牙波登戈犬是两个差异最大的品种。总之，在使用唾液蛋白进行诊断目的时，应考虑刺激程序和犬种对数据分析的影响。
