Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD, 21218, USA.
School of Materials Science and Engineering, Central South University, Changsha, Hunan, 410083, China.
Angew Chem Int Ed Engl. 2017 Mar 27;56(14):3822-3826. doi: 10.1002/anie.201700695. Epub 2017 Feb 15.
Reported is a new shell-based spectroscopic platform, named mechanical trap surface-enhanced Raman spectroscopy (MTSERS), for simultaneous capture, profiling, and 3D microscopic mapping of the intrinsic molecular signatures on the membrane of single live cells. By leveraging the functionalization of the inner surfaces of the MTs with plasmonic gold nanostars, and conformal contact of the cell membrane, MTSERS permits excellent signal enhancement, reliably detects molecular signatures, and allows non-perturbative, multiplex 3D surface imaging of analytes, such as lipids and proteins on the surface of single cells. The demonstrated ability underscores the potential of MTSERS to perform 3D spectroscopic microimaging and to furnish biologically interpretable, quantitative, and dynamic molecular maps in live cell populations.
本文报道了一种基于壳的新型光谱平台，称为机械陷阱表面增强拉曼光谱（MTSERS），用于同时捕获、分析和对单个活细胞膜上的固有分子特征进行 3D 微观映射。通过利用 MT 内表面的等离子体金纳米星的功能化和细胞膜的共形接触，MTSERS 允许出色的信号增强，可靠地检测分子特征，并允许对分析物（如脂质和蛋白质）进行非侵入性、多路复用 3D 表面成像单个细胞表面。所证明的能力强调了 MTSERS 进行 3D 光谱微观成像的潜力，并为活细胞群体提供生物学可解释的、定量的和动态的分子图谱。
